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Literature summary extracted from

  • Hampp, N.; Zenk, M.H.
    Homogeneous strictosidine synthase from cell suspension cultures of Rauvolfia serpentina (1988), Phytochemistry, 27, 3811-3815.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.3.3.2 immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.2 4
-
 tryptamine
-
 Rauvolfia serpentina
4.3.3.2 4
-
 secologanin
-
 Rauvolfia serpentina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.2 30000
-
 gel filtration Rauvolfia serpentina
4.3.3.2 35000
-
 SDS-PAGE Rauvolfia serpentina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 tryptamine + secologanin Rauvolfia serpentina involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.3.3.2 additional information no loss of activity with 5% PEG, dimethylformamide, acetone, ethanol or DMSO Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Rauvolfia serpentina
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.3.3.2 glycoprotein glucose, mannose and xylose bound to enzyme Rauvolfia serpentina

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2
-
 Rauvolfia serpentina

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.2 cell culture
-
 Rauvolfia serpentina
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.2 11.04
-
 purified enzyme Rauvolfia serpentina

Storage Stability

EC Number Storage Stability Organism
4.3.3.2 -20°C, 1000 d, 50% loss of activity Rauvolfia serpentina
4.3.3.2 4°C, 300 d, 50% loss of activity Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 tryptamine + secologanin
-
 Rauvolfia serpentina 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Rauvolfia serpentina 3-alpha(S)-strictosidine
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.3.2 monomer 1 * 35000, SDS-PAGE Rauvolfia serpentina

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.3.3.2 45
-
-
 Rauvolfia serpentina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.3.2 37
-
 immobilized enzyme, half life: 100 d Rauvolfia serpentina

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.3.3.2 4.5
-
 secologanin in reaction with tryptamine Rauvolfia serpentina

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 6.5
-
-
 Rauvolfia serpentina

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.3.2 5 8 50% activity Rauvolfia serpentina