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Literature summary extracted from
- Isoation and immobilization of strictosidine synthase (1987), Methods Enzymol., 136, 342-350.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.3.3.2 | synthesis | immobilized enzyme can be used to synthesize large quantities of 3-alpha(S)-strictosidine, that is a common intermediate in the biosynthesis of indole alkaloids | Catharanthus roseus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.3.3.2 | immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme | Catharanthus roseus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.2 | tryptamine + secologanin | Catharanthus roseus | involved in synthesis of monoterpenoid indole alkaloides | 3-alpha(S)-strictosidine | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.3.2 | Catharanthus roseus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.3.2 | - |
Catharanthus roseus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.3.3.2 | cell culture | - |
Catharanthus roseus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 2.18 | - |
purified enzyme, pH 6.5 | Catharanthus roseus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.2 | tryptamine + secologanin | - |
Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
| 4.3.3.2 | tryptamine + secologanin | involved in synthesis of monoterpenoid indole alkaloides | Catharanthus roseus | 3-alpha(S)-strictosidine | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.3.2 | monomer | - |
Catharanthus roseus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 37 | - |
immobilized enzyme, half life: 68 d, 6% of initial activity after one year | Catharanthus roseus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 6.4 | - |
immobilized enzyme | Catharanthus roseus |
| 4.3.3.2 | 6.8 | - |
soluble enzyme | Catharanthus roseus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.2 | 3 | 9 | immobilized enzyme | Catharanthus roseus |
| 4.3.3.2 | 4 | 8.5 | soluble enzyme | Catharanthus roseus |
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Release 2023.1 (January 2023)
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