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Literature summary extracted from

  • Pfitzner, U.; Zenk, M.H.
    Immobilization of strictosidine synthase from Catharanthus cell cultures and preparative synthesis of strictosidine (1982), Planta Med., 46, 10-14.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.3.2 synthesis immobilized enzyme can be used to synthesize large quantities of 3-alpha(S)-strictosidine, that is a common intermediate in the biosynthesis of indole alkaloids Catharanthus roseus

General Stability

EC Number General Stability Organism
4.3.3.2 immobilized enzyme is more stable to changes in pH and temperature than soluble enzyme Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.2 tryptamine inhibitory above 1 mM, soluble and immobilized enzyme Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.2 0.9
-
 tryptamine immobilized enzyme, pH 6.5, 37°C Catharanthus roseus
4.3.3.2 2.1
-
 secologanin immobilized enzyme, pH 6.5, 37°C Catharanthus roseus
4.3.3.2 2.3
-
 tryptamine soluble enzyme, pH 6.5, 37°C Catharanthus roseus
4.3.3.2 3.4
-
 secologanin soluble enzyme, pH 6.5, 37°C Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 tryptamine + secologanin Catharanthus roseus involved in synthesis of monoterpenoid indole alkaloides 3-alpha(S)-strictosidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2
-
 Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.2 cell culture
-
 Catharanthus roseus
-

Storage Stability

EC Number Storage Stability Organism
4.3.3.2 4°C, immobilized enzyme, 2 years, 55% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 tryptamine + secologanin
-
 Catharanthus roseus 3-alpha(S)-strictosidine
-
 ?
4.3.3.2 tryptamine + secologanin involved in synthesis of monoterpenoid indole alkaloides Catharanthus roseus 3-alpha(S)-strictosidine
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.3.3.2 37
-
 immobilized enzyme, half life: 68 d, 6% of initial activity after one year Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 6.4
-
 immobilized enzyme Catharanthus roseus
4.3.3.2 6.8
-
 soluble enzyme Catharanthus roseus