Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Purmal, A.A.; Rabow, L.E.; Lampman, G.W.; Cunningham, R.P.; Kow, Y.W.
    A common mechanism of action for the N-glycosylase activity of DNA N-glycosylase/AP lyases from E. coli and T4 (1996), Mutat. Res., 364, 193-207.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.99.18 0.0000015
-
 DNA containing thymine glycol pH 7.5 Escherichia coli
4.2.99.18 0.00000167
-
 DNA containing O-benzylhydroxylamine pH 7.5 Escherichia coli
4.2.99.18 0.00000284
-
 DNA containing O-methylhydroxylamine pH 7.5 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.99.18 DNA Escherichia coli
-
 fragments of DNA
-
 ?
4.2.99.18 DNA Tequatrovirus T4
-
 fragments of DNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.99.18 Escherichia coli
-
-
-
4.2.99.18 Tequatrovirus T4
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.99.18 the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate mechanism Escherichia coli
a
4.2.99.18 the C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate mechanism Tequatrovirus T4
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.99.18 DNA
-
 Escherichia coli fragments of DNA
-
 ?
4.2.99.18 DNA
-
 Tequatrovirus T4 fragments of DNA
-
 ?
4.2.99.18 DNA duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates Escherichia coli fragments of DNA
-
 ?
4.2.99.18 DNA duplex oligonucleotides containing the base lesion analogs O-methylhydoxylamine or O-benzylhydroxylamine, N-glycosylase activity generates intermediary AP site which is subsequently cleaved by the enzyme -associated AP lyase activity, O-alkoxyamine-modified AP sites are poorer substrates than the presumed physiological substrates Tequatrovirus T4 fragments of DNA
-
 ?
4.2.99.18 DNA recognizes urea, an oxidative ring fragmentation product of thymine Escherichia coli fragments of DNA
-
 ?
4.2.99.18 DNA containing O-benzylhydroxylamine
-
 Escherichia coli ?
-
 ?
4.2.99.18 DNA containing O-methylhydroxylamine
-
 Escherichia coli ?
-
 ?
4.2.99.18 DNA containing thymine glycol
-
 Escherichia coli ?
-
 ?