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Literature summary extracted from
- Partial purification and characterization of the major endonuclease from Chlamydomonas reinhardtii (1984), Biochim. Biophys. Acta, 782, 170-176.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.18 | dithiothreitol | stimulates over 100% at 1 mM | Chlamydomonas reinhardtii |  |
| 4.2.99.18 | Triton X-100 | 20-50% stimulation at 0.03% | Chlamydomonas reinhardtii | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.18 | KCl | 50 mM: 70% inhibition | Chlamydomonas reinhardtii | |
| 4.2.99.18 | NaCl | 50 mM: 70% inhibition | Chlamydomonas reinhardtii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.99.18 | Mg2+ | Mg2+ or Mn2+ required, Mg2+ better than Mn2+ | Chlamydomonas reinhardtii | |
| 4.2.99.18 | Mn2+ | Mn2+ or Mg2+ required | Chlamydomonas reinhardtii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.18 | 38000 | - |
gel filtration | Chlamydomonas reinhardtii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.18 | DNA | Chlamydomonas reinhardtii | - |
fragments of DNA | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.99.18 | Chlamydomonas reinhardtii | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.99.18 | partial | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.99.18 | DNA | - |
Chlamydomonas reinhardtii | fragments of DNA | - |
? | |
| 4.2.99.18 | DNA | specific for DNA containing either apurinic or apyrimidinic sites | Chlamydomonas reinhardtii | fragments of DNA | - |
? | |
| 4.2.99.18 | additional information | no activity with DNA that has been damaged by UV light, methyl methanesulfonate, osmium teroxide or sodium bisulfite | Chlamydomonas reinhardtii | ? | - |
? | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.99.18 | 7.2 | 8.2 | - |
Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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