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Literature summary extracted from

  • Takikawa, S.; Curtius, H.C.; Redweik, U.; Leimbacher, W.; Ghisla, S.
    Biosynthesis of tetrahydropterin. Purification and characterization of 6-pyruvoyl-tetrahydropterin synthase from human liver (1986), Eur. J. Biochem., 161, 295-302.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.12 dithioerythritol increased catalytic activity with 10 mM Homo sapiens
4.2.3.12 additional information NADPH is not necessary for activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.12 EDTA completely inactivates the enzyme by complexation of Mg2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.01
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens
4.2.3.12 0.01
-
 7,8-dihydroneopterin triphosphate pH 7.5, 37ºC Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 19000
-
 x * 19000, SDS-PAGE Homo sapiens
4.2.3.12 19000
-
 4 * 19000, SDS-PAGE Homo sapiens
4.2.3.12 83000
-
 gel filtration, seems to have 4 identical subunits Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Homo sapiens the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate Homo sapiens assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 ammonium sulfate precipitation, hydroxyapatite column, gel filtration, DEAE-Fractogel 650S column, SDS-PAGE Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 liver
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 additional information
-
-
 Homo sapiens
4.2.3.12 0.1
-
-
 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
4.2.3.12 -20ºC and -70ºC, Tris HCl, several months, stable Homo sapiens
4.2.3.12 -70°C, stable for several months Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the first step in the conversion of 7,8-dihydroneopterin triphosphate to tetrahydrobiopterin Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate
-
 Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ? x * 19000, SDS-PAGE Homo sapiens
4.2.3.12 tetramer 4 * 19000, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.12 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.12 7.5
-
-
 Homo sapiens
4.2.3.12 7.5
-
 in Tris-HCl 100 mM Homo sapiens