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Literature summary extracted from

  • Burgisser, D.M.; Thöny, B.; Redweik, U.; Hunziker, P.; Heizmann, C.W.; Blau, N.
    Expression and characterization of recombinant human and rat liver 6-pyruvoyl tetrahydropterin synthase. Modified cysteine residues inhibit the enzyme activity (1994), Eur. J. Biochem., 219, 497-502.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.12 cloned in Escherichia coli Rattus norvegicus
4.2.3.12 expressed as maltose-binding-6-pyruvoyl-tetrahydropterin-synthase fusion protein Homo sapiens
4.2.3.12 expressed as maltose-binding-6-pyruvoyl-tetrahydropterin-synthase fusion protein Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.12 4-Vinylpyridine 95% inactivation under non-denaturating and non-reducing conditions Homo sapiens
4.2.3.12 4-Vinylpyridine 80% inactivation under non-denaturating and non-reducing conditions Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.008
-
 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin wild-type enzyme Rattus norvegicus
4.2.3.12 0.008
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Rattus norvegicus
4.2.3.12 0.0085
-
 7,8-dihydroneopterin triphosphate pH 7.4, 37ºC Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 15899
-
 3 * 15899, electron-ionization mass spectrometry Rattus norvegicus
4.2.3.12 16000
-
 x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
4.2.3.12 16254
-
 6 * 16254, electron-ionization mass spectrometry Homo sapiens
4.2.3.12 47000
-
 3 * 47000, SDS-PAGE Homo sapiens
4.2.3.12 97000
-
 6 * 97000, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Rattus norvegicus
-
 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Homo sapiens rate-limiting enzyme in the synthesis of human tetrahydrobiopterin 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Homo sapiens
-
-
-
4.2.3.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 affinity chromatography, gel filtration , SDS-PAGE Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 liver
-
 Homo sapiens
-
4.2.3.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 additional information
-
-
 Homo sapiens
4.2.3.12 additional information
-
-
 Rattus norvegicus
4.2.3.12 0.077
-
-
 Homo sapiens
4.2.3.12 0.2766
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Rattus norvegicus 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin rate-limiting enzyme in the synthesis of human tetrahydrobiopterin Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ?
-
 Rattus norvegicus
4.2.3.12 ? x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
4.2.3.12 hexamer
-
 Rattus norvegicus
4.2.3.12 hexamer 6 * 97000, SDS-PAGE Homo sapiens
4.2.3.12 hexamer 6 * 16254, electron-ionization mass spectrometry Homo sapiens
4.2.3.12 trimer 3 * 47000, SDS-PAGE Homo sapiens
4.2.3.12 trimer 3 * 15899, electron-ionization mass spectrometry Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.12 80
-
 stable Homo sapiens
4.2.3.12 80
-
 stable Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.12 additional information
-
 dihydroneopterin triphosphate 0.277 mU/min/mg Rattus norvegicus
4.2.3.12 additional information
-
 dihydroneopterin triphosphate 0.080 mU/min/mg Homo sapiens