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Literature summary extracted from
- Enzymatic properties of 6-pyruvoyl tetrahydropterin synthase purified from fat bodies of silkworm larvae (1993), Adv. Exp. Med. Biol., 338, 191-194.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 4.2.3.12 | medicine | the decrease of this enzyme is the most frequent cause of atypical phenylketonuria | Bombyx mori |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.12 | monoiodoacetate | - |
Bombyx mori |  |
| 4.2.3.12 | NEM | - |
Bombyx mori | |
| 4.2.3.12 | PCMB | - |
Bombyx mori | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.12 | 0.0128 | - |
7,8-dihydroneopterin triphosphate | pH 8.5, 37ºC, 1 mM Mg2+ | Bombyx mori | |
| 4.2.3.12 | 0.0169 | - |
7,8-dihydroneopterin triphosphate | pH 8.5 37ºC, 5 mM Mg2+ | Bombyx mori | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.12 | Ba2+ | stimulatory effect, 5 mM greatly accelerates activity | Bombyx mori | |
| 4.2.3.12 | Ca2+ | stimulatory effect and additive effect at Mg2+, 1 mM | Bombyx mori | |
| 4.2.3.12 | Mg2+ | stimulates, Km of 0.2 mM and 2 mM at pH 8.5 and 37ºC | Bombyx mori | |
| 4.2.3.12 | Mn2+ | stimulatory effect but additional competitive or inhibitory effect | Bombyx mori | |
| 4.2.3.12 | additional information | Cu2+ has no effect on activity | Bombyx mori | |
| 4.2.3.12 | Ni2+ | no effect on enzyme activity | Bombyx mori | |
| 4.2.3.12 | Sr2+ | 5 mM greatly accelerates activity | Bombyx mori | |
| 4.2.3.12 | Zn2+ | no effect on enzyme activity | Bombyx mori | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.12 | 18500 | - |
4 * 18500, SDS-PAGE | Bombyx mori |
| 4.2.3.12 | 72000 | - |
native PAGE | Bombyx mori |
| 4.2.3.12 | 85000 | 87000 | gel filtration | Bombyx mori |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.12 | Bombyx mori | - |
silkworm | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.12 | ammonium sulfate precipitation, hydroxyapatite column, gel filtration | Bombyx mori |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.12 | fat body | larvae | Bombyx mori | - |
| 4.2.3.12 | larva | fat bodies | Bombyx mori | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.12 | 7,8-dihydroneopterin triphosphate | - |
Bombyx mori | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.12 | tetramer | 4 * 18500, SDS-PAGE | Bombyx mori |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.12 | 8.5 | - |
- |
Bombyx mori |
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Release 2023.1 (January 2023)
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