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Literature summary extracted from
- cDNA cloning, expression in Escherichia coli and purification of human 6-pyruvoyl-tetrahydropterin synthase (1993), Biochem. Biophys. Res. Commun., 195, 1386-1393.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.3.12 | cloned in Escherichia coli | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.3.12 | 0.012 | - |
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin | - |
Homo sapiens |  |
| 4.2.3.12 | 0.0124 | - |
7,8-dihydroneopterin triphosphate | 90 KDa protein pH 7.4, 37ºC | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.3.12 | Mg2+ | necessary for activity | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.12 | 17000 | - |
x * 17000, SDS-PAGE | Homo sapiens |
| 4.2.3.12 | 17000 | - |
4 * 17000, SDS-PAGE | Homo sapiens |
| 4.2.3.12 | 90000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.12 | 7,8-dihydroneopterin triphosphate | Homo sapiens | assay at | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.3.12 | Homo sapiens | Q03393 | cloned in Escherichia coli | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.3.12 | affinity chromatography, gel filtration | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.3.12 | MOLT-4 cell | acute lymphoblastic leukemia cell line, 65% homology with rat enzyme | Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.12 | 0.5167 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.3.12 | 7,8-dihydroneopterin triphosphate | - |
Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
| 4.2.3.12 | 7,8-dihydroneopterin triphosphate | assay at | Homo sapiens | 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.3.12 | ? | x * 17000, SDS-PAGE | Homo sapiens |
| 4.2.3.12 | tetramer | 4 * 17000, SDS-PAGE | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.3.12 | 7.5 | 8 | - |
Homo sapiens |
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