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Literature summary extracted from

  • Guzman, J.; Redweik, U.; Schoedon, G.; Hunziker, P.; Wiestler, O.D.; Heizmann, C.W.; Blau, N.
    Purification and characterization of 6-pyruvoyl tetrahydropterin synthase from human pituitary gland (1992), Enzyme, 46, 287-298.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.12 additional information NADPH is not necessary for activity Homo sapiens

General Stability

EC Number General Stability Organism
4.2.3.12 stable at all steps of purification Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.12 additional information L-monapterin does not inhibit activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.12 0.01
-
6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
Homo sapiens
4.2.3.12 0.01
-
7,8-dihydroneopterin triphosphate pH 7.4, 37ºC, shows positive cooperativity Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.12 Mg2+ required Homo sapiens
4.2.3.12 Mg2+ necessary for activity Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 16500
-
4 * 16500, SDS-PAGE Homo sapiens
4.2.3.12 16500
-
x * 16500, SDS-PAGE Homo sapiens
4.2.3.12 68000
-
gel filtration Homo sapiens
4.2.3.12 68000
-
gel filtration Salmo salar

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate Drosophila melanogaster assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate Homo sapiens assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate Salmo salar assay at 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Drosophila melanogaster
-
-
-
4.2.3.12 Homo sapiens
-
-
-
4.2.3.12 Salmo salar
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 ammonium sulfate precipitation, DEAE-Sephadex A50 column Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 head
-
Drosophila melanogaster
-
4.2.3.12 hypophysis
-
Homo sapiens
-
4.2.3.12 liver
-
Homo sapiens
-
4.2.3.12 liver
-
Salmo salar
-
4.2.3.12 pituitary gland heat instability, it is present in the anterior pituitary endothelial cells but not in posterior pituitary Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 40
-
-
Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 7,8-dihydroneopterin triphosphate
-
Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate
-
Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate
-
Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Drosophila melanogaster 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Homo sapiens 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?
4.2.3.12 7,8-dihydroneopterin triphosphate assay at Salmo salar 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate
-
?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ?
-
Drosophila melanogaster
4.2.3.12 ?
-
Salmo salar
4.2.3.12 ? x * 16500, SDS-PAGE Homo sapiens
4.2.3.12 tetramer
-
Salmo salar
4.2.3.12 tetramer 4 * 16500, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.12 37
-
assay at Drosophila melanogaster
4.2.3.12 37
-
assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.12 additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Drosophila melanogaster
4.2.3.12 additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Homo sapiens
4.2.3.12 additional information
-
the human pituitary gland enzyme is heat instable in contrast to the enzyme from human, rat and salmon liver, and Drosophila heads Salmo salar

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.12 7.5
-
-
Drosophila melanogaster
4.2.3.12 7.5
-
-
Homo sapiens
4.2.3.12 7.5
-
-
Salmo salar
4.2.3.12 7.5
-
in Tris-HCl 100 mM Homo sapiens
4.2.3.12 7.5
-
in Tris-HCl 100 mM Salmo salar

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.3.12 NADPH required Homo sapiens