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Literature summary extracted from

  • Thöny, B.; Leimbacher, W.; Blau, N.; Heizmann, C.W.; Burgisser, D.
    Human liver 6-pyruvoyl-tetrahydropterin synthase: expression of the cDNA, purification and preliminary characterization of the recombinant protein (1993), Adv. Exp. Med. Biol., 338, 187-190.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.12 medicine lack of tetrahydrobiopterin leads to hyperphenylalaninemia and a deficiency of biogenic amine neurotransmitters such as dopamine and serotonin and severe progressive mental retardation Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.12 cloned in Escherichia coli Rattus norvegicus
4.2.3.12 expression in Escherichia coli Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.12 Mg2+ required Homo sapiens
4.2.3.12 Mg2+ required Rattus norvegicus
4.2.3.12 Zn2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.12 16000
-
 5 * 16000, SDS-PAGE Homo sapiens
4.2.3.12 16000
-
 x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
4.2.3.12 16257
-
 5 * 16257, electrospray ionization-mass spectrometry Homo sapiens
4.2.3.12 83000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Homo sapiens the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin Rattus norvegicus the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.12 Homo sapiens
-
-
-
4.2.3.12 Rattus norvegicus
-
 cloned in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.12 affinity chromatography, gel filtration, SDS-PAGE Homo sapiens
4.2.3.12 affinity chromatography, gel filtration, SDS-PAGE Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.12 liver
-
 Homo sapiens
-
4.2.3.12 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.12 0.0574
-
-
 Homo sapiens
4.2.3.12 57.4
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin
-
 Rattus norvegicus 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway Homo sapiens 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?
4.2.3.12 6-(L-erythro-1,2-dihydroxypropyl 3-triphosphate)-7,8-dihydropterin the enzyme catalyzes the second step in the tetrahydrobiopterin biosynthetic pathway Rattus norvegicus 6-(1,2-dioxopropyl)-5,6,7,8-tetrahydropterin + triphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.12 ?
-
 Rattus norvegicus
4.2.3.12 ? x * 16000, SDS-PAGE and electrospray ionization-mass spectometry Homo sapiens
4.2.3.12 pentamer 5 * 16000, SDS-PAGE Homo sapiens
4.2.3.12 pentamer 5 * 16257, electrospray ionization-mass spectrometry Homo sapiens