Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hallahan, T.W.; Croteau, R.
    Monoterpene biosynthesis: demonstration of a geranyl pyrophosphate:sabinene hydrate cyclase in soluble enzyme preparations from sweet marjoram (Majorana hortensis) (1988), Arch. Biochem. Biophys., 264, 618-631.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.3.11 diphosphate 0.1 mM: 30% stimulation Origanum majorana

General Stability

EC Number General Stability Organism
4.2.3.11 proteolytic degradation by 0.01 mg/ml trypsin, folowed by addition of soybean trypsin inhibitor: all cyclase activities are destroyed within 10 min, t1/2 is 3 min Origanum majorana

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.11 diphosphate 0.5 mM: 80% inhibition Origanum majorana
4.2.3.11 EDTA
-
 Origanum majorana
4.2.3.11 NaWO4 10 mM, 45% inhibition Origanum majorana
4.2.3.11 NEM IC50: 0.08 mM Origanum majorana
4.2.3.11 NH4VO3 10 mM, 45% inhibition Origanum majorana
4.2.3.11 PCMB IC50: 0.02 mM Origanum majorana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.11 0.0019
-
 geranyl diphosphate pH 7.0, 30°C Origanum majorana
4.2.3.11 0.0105
-
 neryl diphosphate pH 7.0, 30°C Origanum majorana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.11 soluble
-
 Origanum majorana
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.11 Mg2+ Mg2+ or Mn2+ required, Km: 2.4 mM Origanum majorana
4.2.3.11 Mn2+ Mn2+ or Mg2+ required, Km: 0.3 mM Origanum majorana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.11 56000
-
 gel filtration Origanum majorana

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.11 Origanum majorana
-
 Moench
-
4.2.3.11 Origanum majorana Moench
-
 Moench
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.11 leaf
-
 Origanum majorana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.11 geranyl diphosphate
-
 Origanum majorana sabinene hydrate + diphosphate both cis- and trans-isomers of sabinene hydrate are formed ?
4.2.3.11 geranyl diphosphate
-
 Origanum majorana Moench sabinene hydrate + diphosphate both cis- and trans-isomers of sabinene hydrate are formed ?
4.2.3.11 neryl diphosphate
-
 Origanum majorana sabinene hydrate + diphosphate cis- and trans-sabinene hydrate ?
4.2.3.11 neryl diphosphate
-
 Origanum majorana Moench sabinene hydrate + diphosphate cis- and trans-sabinene hydrate ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.3.11 45
-
 complete inactivation after 20 min Origanum majorana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.11 7
-
-
 Origanum majorana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.11 6.3 8 50% of maximal activity at pH 6.3 and 8.0 Origanum majorana

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.3.11 0.02
-
 IC50: 0.02 mM Origanum majorana PCMB
4.2.3.11 0.08
-
 IC50: 0.08 mM Origanum majorana NEM