Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Miyazaki, M.; Obata, J.; Iwamoto, Y.; Oda, T.; Muramatsu, T.
    Calcium-sensitive extracellular poly(alpha-L-guluronate) lyase from a marine bacterium Pseudomonas sp. strain F6: Purification and some properties (2001), Fish. Sci., 67, 956-964.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.11 Guanidinium chloride 8 M urea causes apparent retention of approximately 50% of enzyme activity Pseudomonas sp.
4.2.2.11 SDS 10 mM SDS causes complete loss of activity whereas the ordered beta-structure is created Pseudomonas sp.
4.2.2.11 Urea 8 M urea causes apparent retention of approximately 50% of enzyme activity Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.11 Ca2+ involved in the formation of the active intermediate between the acidic uronates and amino acid side chains of the enzyme Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.11 36000
-
 x * 36000, SDS-PAGE Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.11 Pseudomonas sp.
-
-
-
4.2.2.11 Pseudomonas sp. F6
-
-
-

Subunits

EC Number Subunits Comment Organism
4.2.2.11 ? x * 36000, SDS-PAGE Pseudomonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.11 100
-
 incubation for 15 min causes complete loss of activity, activity diminishes with increasing incubation temperatures below 100 °C, addition of hen egg-white lysozyme decreased its thermal activity dramatically Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.11 7.5
-
-
 Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.11 6.5 8.5 stable Pseudomonas sp.