EC Number | General Stability | Organism |
---|---|---|
4.2.2.11 | more labile to heat treatment in Tris-HCl buffer, pH 7.0 than in phosphate buffer with similar pH | Alteromonas sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | Ba2+ | - |
Alteromonas sp. | |
4.2.2.3 | Cd2+ | - |
Alteromonas sp. | |
4.2.2.3 | Ni2+ | - |
Alteromonas sp. | |
4.2.2.3 | Zn2+ | - |
Alteromonas sp. | |
4.2.2.11 | Ba(OH)2 | slightly inhibitory, percentage of inhibition is dependent on substrate | Alteromonas sp. | |
4.2.2.11 | NiSO4 | slightly inhibitory, percentage of inhibition is dependent on substrate | Alteromonas sp. | |
4.2.2.11 | ZnCl2 | slightly inhibitory, percentage of inhibition is dependent on substrate | Alteromonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.3 | 0.025 | - |
nona-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.028 | - |
octa-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.031 | - |
hepta-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.035 | - |
hexa-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.039 | - |
penta-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.172 | - |
nona-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.186 | - |
octa-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.192 | - |
hepta-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.226 | - |
hexa-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 0.232 | - |
penta-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 3.86 | - |
tetra-alpha1,4-L-guluronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 4.25 | - |
tetra-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.11 | 0.192 | - |
saturated hepta((1-4)-alpha-L-guluronan) | pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH | Alteromonas sp. | |
4.2.2.11 | 0.226 | - |
saturated hexa((1-4)-alpha-L-guluronan) | pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH | Alteromonas sp. | |
4.2.2.11 | 0.232 | - |
saturated penta((1-4)-alpha-L-guluronan) | pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH | Alteromonas sp. | |
4.2.2.11 | 4.25 | - |
saturated tetra((1-4)-alpha-L-guluronan) | pH 8.5, 30 °C, 100 mM glycine in 0.1 N NaCl and 0.1 N NaOH | Alteromonas sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.2.3 | extracellular | secretion to the culture medium | Alteromonas sp. | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.3 | Co2+ | - |
Alteromonas sp. | |
4.2.2.3 | K+ | slightly activating | Alteromonas sp. | |
4.2.2.3 | Mg2+ | slightly activating | Alteromonas sp. | |
4.2.2.3 | Mn2+ | slightly activating | Alteromonas sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 23000 | - |
gel filtration | Alteromonas sp. |
4.2.2.3 | 33900 | - |
1 * 33900, SDS-PAGE | Alteromonas sp. |
4.2.2.11 | 23000 | - |
gel filtration | Alteromonas sp. |
4.2.2.11 | 33900 | - |
x * 33900, SDS-PAGE | Alteromonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.11 | poly-alpha-L-guluronic acid | Alteromonas sp. | - |
unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.3 | Alteromonas sp. | - |
bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase | - |
4.2.2.3 | Alteromonas sp. 272 | - |
bifunctional enzyme, mannuronate- and guluronate-specific alginate lyase | - |
4.2.2.11 | Alteromonas sp. | - |
strain No. 272 | - |
4.2.2.11 | Alteromonas sp. 272 | - |
strain No. 272 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.2.3 | from the culture medium, 135fold, to homogeneity | Alteromonas sp. |
4.2.2.11 | anion exchange chromatography, and Sephadex G-100 chromatography | Alteromonas sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.2.3 | R2-beta-D-mannuronic acid-R1 = R2-OH + 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl-R1 | enzyme seems to have 6 substrate binding sites, also performs reaction of EC 4.2.2.11 | Alteromonas sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.2.3 | culture medium | - |
Alteromonas sp. | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.3 | hepta-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hepta-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hepta-alpha1,4-L-guluronic acid | - |
Alteromonas sp. 272 | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hepta-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | D-mannuronic acid | - |
? | |
4.2.2.3 | hexa-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hexa-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hexa-alpha1,4-L-guluronic acid | - |
Alteromonas sp. 272 | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | hexa-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | D-mannuronic acid | - |
? | |
4.2.2.3 | additional information | no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length | Alteromonas sp. | ? | - |
? | |
4.2.2.3 | additional information | no activity with triguluronate and trimannuronate, but with tetramers or substrates with longer chain length | Alteromonas sp. 272 | ? | - |
? | |
4.2.2.3 | nona-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | nona-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | nona-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | D-mannuronic acid | - |
? | |
4.2.2.3 | octa-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | octa-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | octa-beta1,4-D-mannuronate | - |
Alteromonas sp. | D-mannuronic acid | - |
? | |
4.2.2.3 | octa-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | D-mannuronic acid | - |
? | |
4.2.2.3 | octa-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | D-mannuronic acid + ? | - |
? | |
4.2.2.3 | penta-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | penta-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | penta-alpha1,4-L-guluronic acid | - |
Alteromonas sp. 272 | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | penta-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-oligo-D-mannuronate + D-mannuronate | - |
? | |
4.2.2.3 | poly-alpha1,4-L-guluronic acid | competitive to poly-beta1,4-D-mannuronic acid | Alteromonas sp. | ? | - |
? | |
4.2.2.3 | poly-beta1,4-D-mannuronic acid | competitive to poly-alpha1,4L-guluronic acid | Alteromonas sp. | ? | - |
? | |
4.2.2.3 | sodium alginate | endo-type manner | Alteromonas sp. | ? | - |
? | |
4.2.2.3 | tetra-alpha1,4-L-guluronate | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | tetra-alpha1,4-L-guluronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | tetra-alpha1,4-L-guluronic acid | - |
Alteromonas sp. 272 | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-alpha-1,4-oligo-L-guluronate + L-guluronate | - |
? | |
4.2.2.3 | tetra-beta1,4-D-mannuronic acid | - |
Alteromonas sp. | 4-deoxy-alpha-L-erythro-hex-4-enuronosyl-beta-1,4-D-mannuronoyl-beta-1,4-D-mannuranote + D-mannuronate | - |
? | |
4.2.2.11 | alpha-L-guluronosyl linkage in alginate | endolyase | Alteromonas sp. | ? | - |
? | |
4.2.2.11 | additional information | no action on trimeric guluronan and mannuronan, but on tetramers or more, the enzyme is most likely beta-structure, the subsite number is most likely six for both guluronate and mannuronate units, the catalytic site of this enzyme is located at the midpoint of the subsite | Alteromonas sp. | ? | - |
? | |
4.2.2.11 | poly-alpha-L-guluronic acid | - |
Alteromonas sp. | ? | - |
? | |
4.2.2.11 | poly-alpha-L-guluronic acid | - |
Alteromonas sp. | unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate | - |
? | |
4.2.2.11 | poly-beta1,4-D-mannuronan | competitive to poly-alpha1,4-L-guluronan | Alteromonas sp. | ? | - |
? | |
4.2.2.11 | saturated hepta((1-4)-alpha-L-guluronan) | - |
Alteromonas sp. | ? | - |
? | |
4.2.2.11 | saturated hexa((1-4)-alpha-L-guluronan) | - |
Alteromonas sp. | unsaturated tetramer and a saturated dimer | - |
? | |
4.2.2.11 | saturated penta((1-4)-alpha-L-guluronan) | - |
Alteromonas sp. | ? | - |
? | |
4.2.2.11 | saturated tetra((1-4)-alpha-L-guluronan) | only reactive in a high concentration of enzyme, and for a prolonged reaction time | Alteromonas sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.3 | monomer | 1 * 33900, SDS-PAGE | Alteromonas sp. |
4.2.2.11 | ? | x * 33900, SDS-PAGE | Alteromonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.3 | alginate lyase | - |
Alteromonas sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 30 | - |
assay at | Alteromonas sp. |
4.2.2.11 | 30 | - |
assay at | Alteromonas sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | additional information | - |
- |
Alteromonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.2.3 | 2.5 | - |
tetra-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 3.2 | - |
tetra-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 6.2 | - |
penta-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 7.3 | - |
octa-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 7.4 | - |
penta-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 7.6 | - |
hepta-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 7.8 | - |
nona-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 7.9 | - |
hexa-alpha1,4-L-guluronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 10.8 | - |
nona-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 11.1 | - |
octa-beta1,4-D-mannuronic acid | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 11.9 | - |
hexa-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. | |
4.2.2.3 | 13.2 | - |
hepta-beta1,4-D-mannuronate | pH 8.5, 30°C | Alteromonas sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.3 | 7.5 | 8 | - |
Alteromonas sp. |
4.2.2.11 | 8.5 | - |
assay at | Alteromonas sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 7.5 | 8 | maximal activity, slightly dependent on buffer composition | Alteromonas sp. |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 5 | 11 | stable | Alteromonas sp. |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.3 | Pseudoalteromonas elyakovii | isoelectric focusing | - |
3.8 |
4.2.2.11 | Alteromonas sp. | - |
- |
3.8 |