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Literature summary extracted from

  • Matsubara, Y.; Kawada, R.; Iwasaki, K.; Oda, T.; Muramatsu, T.
    Extracellular poly(alpha-L-guluronate)lyase from Corynebacterium sp.: purification, characteristics, and conformational properties (1998), J. Protein Chem., 17, 29-36.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.11 CuCl2 1 mM, 58% remaining activity Corynebacterium sp.
4.2.2.11 EDTA 1 mM concentration causes 83% inhibition, the enzyme regains about 90% of the original activity when incubated with 10 mM metal compounds such as MnCl2, MgCl2, NiCl2 or CaCl2, the enzyme is most likely to require the metal ions for the expression of activity Corynebacterium sp.
4.2.2.11 FeCl2 1 mM, 29% remaining activity Corynebacterium sp.
4.2.2.11 HgCl2 1 mM, 23% remaining activity Corynebacterium sp.
4.2.2.11 additional information iodoacetate, dithiothreitol, 2-mercaptoethanol, sodium dodecyl sulfate or p-chloromercuribenzoate have no inhibitory effects Corynebacterium sp.
4.2.2.11 Urea enzyme preincubated in the presence of 6 M urea, dialyzed and assayed normally retains 90% activity, but if the enzyme is preincubated and assayed in the presence of 6 M urea no activity is measured Corynebacterium sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.11 extracellular
-
 Corynebacterium sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.11 Ca2+ slight activation at 1 mM or 5 mM concentrations Corynebacterium sp.
4.2.2.11 Mg2+ 1 mM, 11% activation Corynebacterium sp.
4.2.2.11 Mn2+ 5 mM, 255% activity Corynebacterium sp.
4.2.2.11 additional information BaCl2 does not affect enzymatic activity Corynebacterium sp.
4.2.2.11 Ni2+ 1 mM, 13% activation Corynebacterium sp.
4.2.2.11 Zn2+ 1 mM, 175% activity Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.11 27000
-
 gel filtration Corynebacterium sp.
4.2.2.11 28400
-
 amino acid analysis Corynebacterium sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.2.11 poly-alpha-L-guluronic acid Corynebacterium sp.
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid Corynebacterium sp. ALY-1
-
 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.11 Corynebacterium sp.
-
 ALY-1 strain
-
4.2.2.11 Corynebacterium sp. ALY-1
-
 ALY-1 strain
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
4.2.2.11 6M urea collapses the circular dicroic spectral bands of the native enzyme caused by aromatic amino acids side chains and causes dissapearance of the enzyme activity, removal of the denaturant by dialysis restored the native-like spectrum and the activity, but some parts of the enzyme conformation are defective in the renaturation conditions Corynebacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.11 alpha-L-guluronosyl linkage in alginate endolyase Corynebacterium sp. ?
-
 ?
4.2.2.11 alpha-L-guluronosyl linkage in alginate endolyase Corynebacterium sp. ALY-1 ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Corynebacterium sp. ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Corynebacterium sp. ALY-1 ?
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Corynebacterium sp. unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?
4.2.2.11 poly-alpha-L-guluronic acid
-
 Corynebacterium sp. ALY-1 unsaturated 1,4-di-, 1,4-tri- and 1,4-tetrasaccharides of L-guluronate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.11 monomer 1 * 27000, SDS-PAGE Corynebacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.11 55
-
-
 Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.11 7
-
-
 Corynebacterium sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.11 4 10 stable Corynebacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.11 Corynebacterium sp.
-
 7.4 7.3