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Literature summary extracted from
- Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology (1998), Nat. Biotechnol., 16, 733-736.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | light | the enzyme activity is increased by exposure to near ultra-violet light | Rhodococcus sp. |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.84 | expression in Escherichia coli | Pseudomonas chlororaphis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.84 | Co3+ | - |
Pseudomonas putida |  |
| 4.2.1.84 | Fe3+ | - |
Rhodococcus sp. | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.84 | Pseudomonas chlororaphis | - |
- |
- |
| 4.2.1.84 | Pseudomonas chlororaphis B23 | - |
- |
- |
| 4.2.1.84 | Pseudomonas putida | - |
- |
- |
| 4.2.1.84 | Rhodococcus rhodochrous | - |
- |
- |
| 4.2.1.84 | Rhodococcus rhodochrous J1 | - |
- |
- |
| 4.2.1.84 | Rhodococcus sp. | - |
- |
- |
| 4.2.1.84 | Rhodococcus sp. N-774 | - |
- |
- |
| 4.2.1.84 | Rhodococcus sp. R312 | - |
- |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.84 | 2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O | - |
Pseudomonas putida | 2(S)-(4-chloro-phenyl)-3-methyl-butyramide | - |
? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus sp. | pyridine-3-carbamide | - |
? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus rhodochrous | pyridine-3-carbamide | i.e. nicotinamide | ? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus sp. N-774 | pyridine-3-carbamide | - |
? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus sp. R312 | pyridine-3-carbamide | - |
? | |
| 4.2.1.84 | 3-cyanopyridine + H2O | - |
Rhodococcus rhodochrous J1 | pyridine-3-carbamide | i.e. nicotinamide | ? | |
| 4.2.1.84 | acrylamide + H2O | - |
Rhodococcus rhodochrous | acrylonitrile | - |
? | |
| 4.2.1.84 | acrylamide + H2O | - |
Rhodococcus sp. | acrylonitrile | - |
? | |
| 4.2.1.84 | acrylamide + H2O | - |
Rhodococcus sp. N-774 | acrylonitrile | - |
? | |
| 4.2.1.84 | acrylamide + H2O | - |
Rhodococcus sp. R312 | acrylonitrile | - |
? | |
| 4.2.1.84 | acrylamide + H2O | - |
Rhodococcus rhodochrous J1 | acrylonitrile | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.84 | dimer | - |
Pseudomonas putida |
| 4.2.1.84 | dimer | - |
Rhodococcus sp. |
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