BRENDA - Enzyme Database show

Metalloenzyme nitrile hydratase: structure, regulation, and application to biotechnology

Kobayashi, M.; Shimizu, S.; Nat. Biotechnol. 16, 733-736 (1998)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.84
light
the enzyme activity is increased by exposure to near ultra-violet light
Rhodococcus sp.
Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.84
expression in Escherichia coli
Pseudomonas chlororaphis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.84
Co3+
-
Pseudomonas putida
4.2.1.84
Fe3+
-
Rhodococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.84
Pseudomonas chlororaphis
-
-
-
4.2.1.84
Pseudomonas chlororaphis B23
-
-
-
4.2.1.84
Pseudomonas putida
-
-
-
4.2.1.84
Rhodococcus rhodochrous
-
-
-
4.2.1.84
Rhodococcus rhodochrous J1
-
-
-
4.2.1.84
Rhodococcus sp.
-
-
-
4.2.1.84
Rhodococcus sp. N-774
-
-
-
4.2.1.84
Rhodococcus sp. R312
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.84
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
648454
Pseudomonas putida
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
648454
Pseudomonas putida
?
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp.
pyridine-3-carbamide
-
648454
Rhodococcus sp.
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648454
Rhodococcus rhodochrous
?
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp. N-774
pyridine-3-carbamide
-
648454
Rhodococcus sp. N-774
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp. R312
pyridine-3-carbamide
-
648454
Rhodococcus sp. R312
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus rhodochrous J1
pyridine-3-carbamide
i.e. nicotinamide
648454
Rhodococcus rhodochrous J1
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus rhodochrous
acrylonitrile
-
-
-
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp.
acrylonitrile
-
648454
Rhodococcus sp.
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp. N-774
acrylonitrile
-
648454
Rhodococcus sp. N-774
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp. R312
acrylonitrile
-
648454
Rhodococcus sp. R312
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus rhodochrous J1
acrylonitrile
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.84
dimer
-
Pseudomonas putida
4.2.1.84
dimer
-
Rhodococcus sp.
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.2.1.84
light
the enzyme activity is increased by exposure to near ultra-violet light
Rhodococcus sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.84
expression in Escherichia coli
Pseudomonas chlororaphis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.84
Co3+
-
Pseudomonas putida
4.2.1.84
Fe3+
-
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.84
2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
648454
Pseudomonas putida
2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
648454
Pseudomonas putida
?
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp.
pyridine-3-carbamide
-
648454
Rhodococcus sp.
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648454
Rhodococcus rhodochrous
?
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp. N-774
pyridine-3-carbamide
-
648454
Rhodococcus sp. N-774
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus sp. R312
pyridine-3-carbamide
-
648454
Rhodococcus sp. R312
-
4.2.1.84
3-cyanopyridine + H2O
-
648454
Rhodococcus rhodochrous J1
pyridine-3-carbamide
i.e. nicotinamide
648454
Rhodococcus rhodochrous J1
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus rhodochrous
acrylonitrile
-
-
-
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp.
acrylonitrile
-
648454
Rhodococcus sp.
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp. N-774
acrylonitrile
-
648454
Rhodococcus sp. N-774
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus sp. R312
acrylonitrile
-
648454
Rhodococcus sp. R312
?
4.2.1.84
acrylamide + H2O
-
648454
Rhodococcus rhodochrous J1
acrylonitrile
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.84
dimer
-
Pseudomonas putida
4.2.1.84
dimer
-
Rhodococcus sp.