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Literature summary extracted from

  • Wu, S.; Fallon, R.D.; Payne, M.S.
    Over-production of stereoselective nitrile hydratase from Pseudomonas putida 5B in Escherichia coli: activity requires a novel downstream protein (1997), Appl. Microbiol. Biotechnol., 48, 704-708.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.84 P14 protein protein encoded in an open frame of the structural frames that is essential for optimal activity of NHase over-produced in Escherichia coli Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84 expression in Escherichia coli, strain BL21(DE3) Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 22982
-
 1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Pseudomonas putida
-
-
-
4.2.1.84 Pseudomonas putida 5B
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida 2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 2(R)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida 5B 2(R)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
 Pseudomonas putida 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O
-
 Pseudomonas putida 5B 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 additional information S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion Pseudomonas putida ?
-
 ?
4.2.1.84 additional information S enantiomer conversion is 14 times greater than the rate of R enantiomer conversion Pseudomonas putida 5B ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 dimer 1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida