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Literature summary extracted from

  • Payne, M.S.; Wu, S.; Fallon, R.D.; Tudor, G.; Stieglitz, B.; Turner, I.M., Jr.; Nelson, M.J.
    A stereoselective cobalt-containing nitrile hydratase (1997), Biochemistry, 36, 5447-5454.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.84
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 16
-
 Methacrylonitrile pH 7.2, 25ºC Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Co3+ CD spectrum suggests low-spin Co3+ in tetragonally-distorted octahedral ligand field Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 22982
-
 1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida
4.2.1.84 22982
-
 2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration Pseudomonas putida
4.2.1.84 24108
-
 2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration Pseudomonas putida
4.2.1.84 54000
-
 chromatography on Sephacryl S-400 and Sepharose CL-6B, alpha-beta form Pseudomonas putida
4.2.1.84 94000
-
 gel chromatography on Superdex 200 , (alpha-beta)2 form Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Pseudomonas putida
-
-
-
4.2.1.84 Pseudomonas putida NRRL-18668
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 chromatography on DEAE-Cellulose, hydroxyapatite, phenyl-Sepharose and Sephacryl S-400 Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 1590
-
 purified enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 2(S)-(4-chlorophenyl)-3-methylbutyronitrile + H2O enantioselective hydration Pseudomonas putida NRRL-18668 2(S)-(4-chloro-phenyl)-3-methyl-butyramide
-
 ?
4.2.1.84 methacrylonitrile + H2O
-
 Pseudomonas putida methylacrylic acid amide
-
 ?
4.2.1.84 methacrylonitrile + H2O
-
 Pseudomonas putida NRRL-18668 methylacrylic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 dimer gel filtration Pseudomonas putida
4.2.1.84 dimer 1 * 22982, alpha + 1* 24108, beta, amino acid sequence Pseudomonas putida
4.2.1.84 tetramer 2 * 22982, alpha + 2 * 24108, beta, amino acid sequence, gel filtration Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 35
-
 1 hour, 10 mM methacrylonitrile stable up to this temperature Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7.2 7.8
-
 Pseudomonas putida