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Literature summary extracted from

  • Kopf, M-A.; Bonnet, D.; Artaud, I.; Pétré, D.; Mansuy, D.
    Key role of alkanoic acids on the spectral properties, activity, and active-site stability of iron-containing nitrile hydratase from Brevibacterium R312 (1996), Eur. J. Biochem., 240, 239-244.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 adipic acid not inhibitory Brevibacterium sp.
4.2.1.84 hexanoic acid IC50 of 2 mM Brevibacterium sp.
4.2.1.84 additional information maleic, succinic, glutaric and pimelic acid do not act as inhibitors Brevibacterium sp.
4.2.1.84 n-butyric acid competitive inhibitor. Complete inhibition beyond 40 mM. IC50 of 4 mM at pH 7.2 in Hepes buffer 100 mM, IC50 of 8 mM at pH 7.2 in sodium phosphate buffer 100 mM, IC50 of 0.8 mM at pH 6.2 in sodium phosphate buffer 100 mM, IC50 of 12 mM at pH 8.2 in sodium phosphate buffer 100 mM Brevibacterium sp.
4.2.1.84 Valeric acid IC50 of 0.5 mM Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 17
-
acrylonitrile pH 7.2, 28ºC, L-NHase Brevibacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe2+ two mol iron/mol of enzyme Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 95000
-
4 * 95000 Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Brevibacterium sp.
-
-
-
4.2.1.84 Brevibacterium sp. R312
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 1325
-
in the presence of 0.035 mM n-butyric acid Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acrylonitrile + H2O
-
Brevibacterium sp. 2-propenoic acid amide
-
?
4.2.1.84 acrylonitrile + H2O
-
Brevibacterium sp. R312 2-propenoic acid amide
-
?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 tetramer 4 * 95000 Brevibacterium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.9
-
n-butyric acid pH 7.2, 28ºC Brevibacterium sp.

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.2.1.84 0.5
-
IC50 of 0.5 mM Brevibacterium sp. Valeric acid
4.2.1.84 2
-
IC50 of 2 mM Brevibacterium sp. hexanoic acid
4.2.1.84 12
-
competitive inhibitor. Complete inhibition beyond 40 mM. IC50 of 4 mM at pH 7.2 in Hepes buffer 100 mM, IC50 of 8 mM at pH 7.2 in sodium phosphate buffer 100 mM, IC50 of 0.8 mM at pH 6.2 in sodium phosphate buffer 100 mM, IC50 of 12 mM at pH 8.2 in sodium Brevibacterium sp. n-butyric acid