Data extracted from this reference:
Activating Compound
4.2.1.84
crotonamide
induces high and low molecular mass forms of the enzyme
Rhodococcus rhodochrous
4.2.1.84
cyclohexanecarboxamide
induces the low molecular mass form of the enzyme
Rhodococcus rhodochrous
4.2.1.84
light
the enzyme activity is increased by exposure to near ultra-violet light
Rhodococcus sp.
4.2.1.84
Urea
induces the high molecular mass form of the enzyme
Rhodococcus rhodochrous
Cloned(Commentary)
4.2.1.84
Albifimbria verrucaria
4.2.1.84
expression in Escherichia coli
Rhodococcus sp.
4.2.1.84
high molecular mass-NHase and low molecular mass-NHase genes cloned into Escherichia coli
Rhodococcus rhodochrous
General Stability
4.2.1.84
unstable when diluted, but completely stabilized with low-molecular organic acids such as n-butyric acid, n-valeric acid, propionic acid and acetic acid
Pseudomonas chlororaphis
Inhibitors
4.2.1.84
additional information
high concentrations of acrylonitrile inhibit the enzyme activity
Pseudomonas chlororaphis
KM Value [mM]
4.2.1.84
0.001
phenylacetonitrile
Agrobacterium tumefaciens
4.2.1.84
0.0079
indole-3-acetonitrile
Agrobacterium tumefaciens
4.2.1.84
1.89
acrylonitrile
Rhodococcus rhodochrous
Metals/Ions
4.2.1.84
Co2+
Rhodococcus sp.
4.2.1.84
Co2+
cannot replace ferrous or ferric ions
Pseudomonas chlororaphis
4.2.1.84
Co2+
indispensable for high enzyme activity; induces the enzyme activity
Rhodococcus rhodochrous
4.2.1.84
Fe2+
Rhodococcus rhodochrous
4.2.1.84
Fe2+
non-heme iron enzyme with a typical low-spin Fe(III)-active center
Rhodococcus sp.
4.2.1.84
Fe2+
ferrous or ferric ions greatly increase the enzyme activity; non-heme iron enzyme with a typical low-spin Fe(III)-active center
Pseudomonas chlororaphis
Molecular Weight [Da]
4.2.1.84
27700
2 * 27700
Albifimbria verrucaria
4.2.1.84
130000
gel filtration, low molecular mass enzyme, L-NHase
Rhodococcus rhodochrous
4.2.1.84
520000
high molecular mass enzyme, H-NHase
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates)
4.2.1.84
indole-3-acetonitrile + H2O
Agrobacterium tumefaciens
(indole-3-yl)acetamide
Agrobacterium tumefaciens
?
4.2.1.84
phenylacetonitrile + 2 H2O
Agrobacterium tumefaciens
phenylacetic acid + NH3
Agrobacterium tumefaciens
?
Organism
4.2.1.84
Agrobacterium tumefaciens
4.2.1.84
Albifimbria verrucaria
4.2.1.84
Arthrobacter sp.
4.2.1.84
Pseudomonas chlororaphis
4.2.1.84
Pseudomonas chlororaphis B23
4.2.1.84
Rhodococcus rhodochrous
; J1
4.2.1.84
Rhodococcus rhodochrous J1
; J1
Specific Activity [micromol/min/mg]
4.2.1.84
additional information
141 U/mg of dry cells, Am 324 mutant strain
Pseudomonas chlororaphis
4.2.1.84
additional information
Rhodococcus rhodochrous
4.2.1.84
additional information
Rhodococcus sp.
Substrates and Products (Substrate)
4.2.1.84
3-cyanopyridine + H2O
648444
Rhodococcus sp.
pyridine-3-carbamide
648444
Rhodococcus sp.
4.2.1.84
3-cyanopyridine + H2O
648444
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648444
Rhodococcus rhodochrous
?
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus rhodochrous
acetamide
?
4.2.1.84
acetonitrile + H2O
648444
Arthrobacter sp.
acetamide
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus sp.
acetamide
?
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus rhodochrous J1
acetamide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Arthrobacter sp.
2-propenoic acid amide
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus sp.
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Pseudomonas chlororaphis
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Pseudomonas chlororaphis B23
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous J1
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous
acrylamide
4.2.1.84
cyanamide + H2O
648444
Albifimbria verrucaria
urea
648444
Albifimbria verrucaria
?
4.2.1.84
hydroxyacetonitrile + H2O
648444
Arthrobacter sp.
hydroxyacetamide
648444
Arthrobacter sp.
4.2.1.84
indole-3-acetonitrile + H2O
648444
Agrobacterium tumefaciens
(indole-3-yl)acetamide
648444
Agrobacterium tumefaciens
?
4.2.1.84
methacrylamide + H2O
causes the greatest induction of activity
648444
Pseudomonas chlororaphis
?
?
4.2.1.84
methacrylamide + H2O
causes the greatest induction of activity
648444
Pseudomonas chlororaphis B23
?
?
4.2.1.84
additional information
648444
Pseudomonas chlororaphis
?
4.2.1.84
additional information
the enzyme acts on low-molecular aliphatic nitriles with 2-5 carbons but not on aliphatic nitriles with more than 6 carbons
648444
Arthrobacter sp.
?
4.2.1.84
additional information
H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles
648444
Rhodococcus rhodochrous
?
4.2.1.84
additional information
648444
Pseudomonas chlororaphis B23
?
4.2.1.84
additional information
H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles
648444
Rhodococcus rhodochrous J1
?
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus rhodochrous
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Arthrobacter sp.
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus sp.
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Pseudomonas chlororaphis
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Pseudomonas chlororaphis B23
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus rhodochrous J1
n-butyric acid amide
4.2.1.84
phenylacetonitrile + 2 H2O
648444
Agrobacterium tumefaciens
phenylacetic acid + NH3
648444
Agrobacterium tumefaciens
?
4.2.1.84
propionitrile + H2O
648444
Rhodococcus rhodochrous
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Arthrobacter sp.
propionic acid amide
4.2.1.84
propionitrile + H2O
648444
Rhodococcus sp.
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Pseudomonas chlororaphis
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Pseudomonas chlororaphis B23
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Rhodococcus rhodochrous J1
propionic acid amide
?
Subunits
4.2.1.84
dimer
Rhodococcus sp.
4.2.1.84
dimer
2 * 27700
Albifimbria verrucaria
Temperature Optimum [°C]
4.2.1.84
5
Rhodococcus rhodochrous
Temperature Stability [°C]
4.2.1.84
additional information
the enzyme is heat-stable
Rhodococcus rhodochrous
pH Optimum
4.2.1.84
7
Rhodococcus rhodochrous
Cofactor
4.2.1.84
pyrroloquinoline quinone
Pseudomonas chlororaphis
Activating Compound (protein specific)
4.2.1.84
crotonamide
induces high and low molecular mass forms of the enzyme
Rhodococcus rhodochrous
4.2.1.84
cyclohexanecarboxamide
induces the low molecular mass form of the enzyme
Rhodococcus rhodochrous
4.2.1.84
light
the enzyme activity is increased by exposure to near ultra-violet light
Rhodococcus sp.
4.2.1.84
Urea
induces the high molecular mass form of the enzyme
Rhodococcus rhodochrous
Cloned(Commentary) (protein specific)
4.2.1.84
Albifimbria verrucaria
4.2.1.84
expression in Escherichia coli
Rhodococcus sp.
4.2.1.84
high molecular mass-NHase and low molecular mass-NHase genes cloned into Escherichia coli
Rhodococcus rhodochrous
Cofactor (protein specific)
4.2.1.84
pyrroloquinoline quinone
Pseudomonas chlororaphis
General Stability (protein specific)
4.2.1.84
unstable when diluted, but completely stabilized with low-molecular organic acids such as n-butyric acid, n-valeric acid, propionic acid and acetic acid
Pseudomonas chlororaphis
Inhibitors (protein specific)
4.2.1.84
additional information
high concentrations of acrylonitrile inhibit the enzyme activity
Pseudomonas chlororaphis
KM Value [mM] (protein specific)
4.2.1.84
0.001
phenylacetonitrile
Agrobacterium tumefaciens
4.2.1.84
0.0079
indole-3-acetonitrile
Agrobacterium tumefaciens
4.2.1.84
1.89
acrylonitrile
Rhodococcus rhodochrous
Metals/Ions (protein specific)
4.2.1.84
Co2+
Rhodococcus sp.
4.2.1.84
Co2+
cannot replace ferrous or ferric ions
Pseudomonas chlororaphis
4.2.1.84
Co2+
indispensable for high enzyme activity; induces the enzyme activity
Rhodococcus rhodochrous
4.2.1.84
Fe2+
Rhodococcus rhodochrous
4.2.1.84
Fe2+
non-heme iron enzyme with a typical low-spin Fe(III)-active center
Rhodococcus sp.
4.2.1.84
Fe2+
ferrous or ferric ions greatly increase the enzyme activity; non-heme iron enzyme with a typical low-spin Fe(III)-active center
Pseudomonas chlororaphis
Molecular Weight [Da] (protein specific)
4.2.1.84
27700
2 * 27700
Albifimbria verrucaria
4.2.1.84
130000
gel filtration, low molecular mass enzyme, L-NHase
Rhodococcus rhodochrous
4.2.1.84
520000
high molecular mass enzyme, H-NHase
Rhodococcus rhodochrous
Natural Substrates/ Products (Substrates) (protein specific)
4.2.1.84
indole-3-acetonitrile + H2O
Agrobacterium tumefaciens
(indole-3-yl)acetamide
Agrobacterium tumefaciens
?
4.2.1.84
phenylacetonitrile + 2 H2O
Agrobacterium tumefaciens
phenylacetic acid + NH3
Agrobacterium tumefaciens
?
Specific Activity [micromol/min/mg] (protein specific)
4.2.1.84
additional information
141 U/mg of dry cells, Am 324 mutant strain
Pseudomonas chlororaphis
4.2.1.84
additional information
Rhodococcus rhodochrous
4.2.1.84
additional information
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
4.2.1.84
3-cyanopyridine + H2O
648444
Rhodococcus sp.
pyridine-3-carbamide
648444
Rhodococcus sp.
4.2.1.84
3-cyanopyridine + H2O
648444
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648444
Rhodococcus rhodochrous
?
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus rhodochrous
acetamide
?
4.2.1.84
acetonitrile + H2O
648444
Arthrobacter sp.
acetamide
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus sp.
acetamide
?
4.2.1.84
acetonitrile + H2O
648444
Rhodococcus rhodochrous J1
acetamide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Arthrobacter sp.
2-propenoic acid amide
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus sp.
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Pseudomonas chlororaphis
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Pseudomonas chlororaphis B23
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous J1
2-propenoic acid amide
?
4.2.1.84
acrylonitrile + H2O
648444
Rhodococcus rhodochrous
acrylamide
4.2.1.84
cyanamide + H2O
648444
Albifimbria verrucaria
urea
648444
Albifimbria verrucaria
?
4.2.1.84
hydroxyacetonitrile + H2O
648444
Arthrobacter sp.
hydroxyacetamide
648444
Arthrobacter sp.
4.2.1.84
indole-3-acetonitrile + H2O
648444
Agrobacterium tumefaciens
(indole-3-yl)acetamide
648444
Agrobacterium tumefaciens
?
4.2.1.84
methacrylamide + H2O
causes the greatest induction of activity
648444
Pseudomonas chlororaphis
?
?
4.2.1.84
methacrylamide + H2O
causes the greatest induction of activity
648444
Pseudomonas chlororaphis B23
?
?
4.2.1.84
additional information
648444
Pseudomonas chlororaphis
?
4.2.1.84
additional information
the enzyme acts on low-molecular aliphatic nitriles with 2-5 carbons but not on aliphatic nitriles with more than 6 carbons
648444
Arthrobacter sp.
?
4.2.1.84
additional information
H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles
648444
Rhodococcus rhodochrous
?
4.2.1.84
additional information
648444
Pseudomonas chlororaphis B23
?
4.2.1.84
additional information
H-NHase acts preferentially on aliphatic nitriles, while L-NHase has a higher affinity for aromatic nitriles
648444
Rhodococcus rhodochrous J1
?
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus rhodochrous
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Arthrobacter sp.
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus sp.
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Pseudomonas chlororaphis
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Pseudomonas chlororaphis B23
n-butyric acid amide
4.2.1.84
n-butyronitrile + H2O
648444
Rhodococcus rhodochrous J1
n-butyric acid amide
4.2.1.84
phenylacetonitrile + 2 H2O
648444
Agrobacterium tumefaciens
phenylacetic acid + NH3
648444
Agrobacterium tumefaciens
?
4.2.1.84
propionitrile + H2O
648444
Rhodococcus rhodochrous
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Arthrobacter sp.
propionic acid amide
4.2.1.84
propionitrile + H2O
648444
Rhodococcus sp.
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Pseudomonas chlororaphis
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Pseudomonas chlororaphis B23
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648444
Rhodococcus rhodochrous J1
propionic acid amide
?
Subunits (protein specific)
4.2.1.84
dimer
Rhodococcus sp.
4.2.1.84
dimer
2 * 27700
Albifimbria verrucaria
Temperature Optimum [°C] (protein specific)
4.2.1.84
5
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
4.2.1.84
additional information
the enzyme is heat-stable
Rhodococcus rhodochrous
pH Optimum (protein specific)
4.2.1.84
7
Rhodococcus rhodochrous