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Literature summary extracted from

  • Tani, Y.; Kurihara, M.; Nishise, H.
    Characterization of nitrile hydratase and amidase, which are responsible for the conversion of dinitriles to mononitriles from Corynebacterium sp. (1989), Agric. Biol. Chem., 53, 3151-3158.
No PubMed abstract available

General Stability

EC Number General Stability Organism
4.2.1.84 the enzyme becomes more unstable as it becomes purer. Isovalerate, 30 mM, and caprylate stabilize effectively Corynebacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 1,10-phenanthroline 1 mM Corynebacterium sp.
4.2.1.84 Cu2+ 1 mM CuCl2 Corynebacterium sp.
4.2.1.84 FeCl3 1 mM Corynebacterium sp.
4.2.1.84 FeSO4 1 mM Corynebacterium sp.
4.2.1.84 Hg2+ HgCl2, 0.01-1 mM Corynebacterium sp.
4.2.1.84 hydrazine 1 mM Corynebacterium sp.
4.2.1.84 KCN weak inhibition at 1 mM Corynebacterium sp.
4.2.1.84 Mn2+ MnCl2, 1 mM Corynebacterium sp.
4.2.1.84 NiCl2 1 mM Corynebacterium sp.
4.2.1.84 p-chloromercuribenzoate 1 mM Corynebacterium sp.
4.2.1.84 phenylhydrazine 1 mM Corynebacterium sp.
4.2.1.84 sulfhydryl reagents 1 mM Corynebacterium sp.
4.2.1.84 Zn2+ ZnCl2, 1 mM Corynebacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 3.06
-
 trans-4-Cyanocyclohexane-1-carboxylic acid
-
 Corynebacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe2+ the enzyme contains iron Corynebacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 26900
-
 2 * 26900, SDS-PAGE Corynebacterium sp.
4.2.1.84 61400
-
 gel filtration Corynebacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Corynebacterium sp.
-
 C5
-
4.2.1.84 Corynebacterium sp. C5
-
 C5
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 chromatography on DEAE-Cellulose, DEAE-Sephacel and phenyl-Sepharose CL-4B, 130fold purification Corynebacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
-
 Corynebacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 4-cyanobenzoic acid + H2O
-
 Corynebacterium sp. 4-(aminocarbonyl)benzoic acid
-
 ?
4.2.1.84 4-hydroxybenzonitrile + H2O
-
 Corynebacterium sp. 4-hydroxybenzoic acid amide
-
 ?
4.2.1.84 acetonitrile + H2O
-
 Corynebacterium sp. acetamide
-
 ?
4.2.1.84 acetonitrile + H2O
-
 Corynebacterium sp. C5 acetamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Corynebacterium sp. 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 benzonitrile + H2O
-
 Corynebacterium sp. benzoic acid amide
-
 ?
4.2.1.84 chloroacetonitrile + H2O
-
 Corynebacterium sp. chloroacetamide
-
 ?
4.2.1.84 chloroacetonitrile + H2O
-
 Corynebacterium sp. C5 chloroacetamide
-
 ?
4.2.1.84 crotononitrile + H2O 19% of the activity with propionitrile Corynebacterium sp. (E)-2-butenoic acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O
-
 Corynebacterium sp. isobutyric acid amide
-
 ?
4.2.1.84 isovaleronitrile + H2O more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. isovaleric acid amide
-
 ?
4.2.1.84 methacrylonitrile + H2O more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. methylacrylic acid amide
-
 ?
4.2.1.84 n-butyronitrile + H2O
-
 Corynebacterium sp. n-butyric acid amide
-
 ?
4.2.1.84 n-butyronitrile + H2O
-
 Corynebacterium sp. C5 n-butyric acid amide
-
 ?
4.2.1.84 n-capronitrile + H2O
-
 Corynebacterium sp. n-hexanoic acid amide
-
 ?
4.2.1.84 pivalonitrile + H2O
-
 Corynebacterium sp. 2,2-dimethylpropionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Corynebacterium sp. propionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Corynebacterium sp. C5 propionic acid amide
-
 ?
4.2.1.84 trans-4-cyanocyclohexane-1-carboxylic acid + H2O
-
 Corynebacterium sp. 4-(aminocarbonyl)cyclohexanecarboxylic acid
-
 ?
4.2.1.84 trans-4-cyanocyclohexane-1-carboxylic acid + H2O
-
 Corynebacterium sp. C5 4-(aminocarbonyl)cyclohexanecarboxylic acid
-
 ?
4.2.1.84 valeronitrile + H2O more than 3 times more active than trans-4-cyanocyclohexane-1-carboxylic acid as substrate Corynebacterium sp. n-pentanoic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 dimer 2 * 26900, SDS-PAGE Corynebacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 10
-
 pH 7.5, 10 min, stable Corynebacterium sp.
4.2.1.84 40
-
 pH 7.5, 10 min, stable Corynebacterium sp.
4.2.1.84 45
-
 pH 7.5, 10 min, 60% loss of activity Corynebacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 8 8.5
-
 Corynebacterium sp.