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Literature summary extracted from

  • Moreau, J.L.; Azza, S.; Arnaud, A.; Galzy, P.
    Purification and characterization of nitrile hydratase of a mutant strain of Brevibacterium sp. (1993), J. Basic Microbiol., 33, 323-329.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 Adipamic acid
-
 Brevibacterium sp.
4.2.1.84 adipamide
-
 Brevibacterium sp.
4.2.1.84 adipic acid
-
 Brevibacterium sp.
4.2.1.84 Ca2+ weak Brevibacterium sp.
4.2.1.84 Cu2+ 85% inhibition at 10 mM Brevibacterium sp.
4.2.1.84 Cyanovaleramide
-
 Brevibacterium sp.
4.2.1.84 Cyanovaleric acid
-
 Brevibacterium sp.
4.2.1.84 diisopropylfluorophosphate 0.05 mM, 100% inhibition Brevibacterium sp.
4.2.1.84 Glutaraldehyde 0.05 mM, 58% inhibition Brevibacterium sp.
4.2.1.84 Hg2+ 10 mM, 90% inhibition Brevibacterium sp.
4.2.1.84 iodoacetic acid at 0.05 mM, 27% inhibition Brevibacterium sp.
4.2.1.84 Mn2+
-
 Brevibacterium sp.
4.2.1.84 N,N-Dimethylacetamide
-
 Brevibacterium sp.
4.2.1.84 N-bromosuccinimide 100% inhibition at 0.005 mM Brevibacterium sp.
4.2.1.84 NEM 68% inhibition at 0.05 mM Brevibacterium sp.
4.2.1.84 Thioacetamide
-
 Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 additional information
-
 additional information
-
 Brevibacterium sp.
4.2.1.84 8
-
 Propionitrile R312 strain Brevibacterium sp.
4.2.1.84 21
-
 Cyanovaleric acid ACV2 mutant Brevibacterium sp.
4.2.1.84 28
-
 Cyanovaleric acid R312 strain Brevibacterium sp.
4.2.1.84 30
-
 adiponitrile R312 strain Brevibacterium sp.
4.2.1.84 38
-
 adiponitrile ACV2 mutant Brevibacterium sp.
4.2.1.84 50
-
 Propionitrile ACV2 mutant Brevibacterium sp.
4.2.1.84 156
-
 Cyanovaleramide R312 strain Brevibacterium sp.
4.2.1.84 370
-
 Cyanovaleramide ACV2 mutant Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 26000
-
 x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.
4.2.1.84 27500
-
 x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.
4.2.1.84 80000
-
 gel filtration Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Brevibacterium sp.
-
-
-
4.2.1.84 Brevibacterium sp.
-
 mutant strain ACV2
-
4.2.1.84 Brevibacterium sp. R312
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 ammonium sulfate precipitation, ion exchange chromatography and chromatography on phenyl-Sepharose CL-4B, 11fold purification, mutant strain ACV2 Brevibacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 1600
-
 purified enzyme Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 adiponitrile + 2 H2O
-
 Brevibacterium sp. adipic acid amide
-
 ?
4.2.1.84 adiponitrile + 2 H2O
-
 Brevibacterium sp. R312 adipic acid amide
-
 ?
4.2.1.84 cyanovaleramide
-
 Brevibacterium sp. valerodinitrile + H2O
-
 ?
4.2.1.84 cyanovaleramide
-
 Brevibacterium sp. R312 valerodinitrile + H2O
-
 ?
4.2.1.84 cyanovaleric acid + H2O
-
 Brevibacterium sp. ?
-
 ?
4.2.1.84 cyanovaleric acid + H2O
-
 Brevibacterium sp. R312 ?
-
 ?
4.2.1.84 additional information wide substrate spectrum Brevibacterium sp. ?
-
 ?
4.2.1.84 additional information wide substrate spectrum Brevibacterium sp. R312 ?
-
 ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. propionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. R312 propionic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 26000 + x * 27500, SDS-PAGE Brevibacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 35
-
-
 Brevibacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 45
-
 5 min, complete loss of activity Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 6
-
-
 Brevibacterium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.35
-
 Cyanovaleric acid R312 strain Brevibacterium sp.
4.2.1.84 2
-
 N,N-Dimethylacetamide R312 strain Brevibacterium sp.
4.2.1.84 2
-
 N,N-Dimethylacetamide ACV2 mutant Brevibacterium sp.
4.2.1.84 3.8
-
 Adipamic acid ACV2 mutant Brevibacterium sp.
4.2.1.84 5
-
 Cyanovaleric acid ACV2 mutant Brevibacterium sp.
4.2.1.84 12
-
 Cyanovaleramide R312 strain Brevibacterium sp.
4.2.1.84 14.5
-
 adipamide ACV2 mutant Brevibacterium sp.
4.2.1.84 31
-
 adipamide R312 strain Brevibacterium sp.
4.2.1.84 35
-
 adipic acid R312 strain Brevibacterium sp.
4.2.1.84 43
-
 Adipamic acid R312 strain Brevibacterium sp.
4.2.1.84 73
-
 Cyanovaleramide ACV2 mutant Brevibacterium sp.
4.2.1.84 100
-
 Thioacetamide R312 strain Brevibacterium sp.
4.2.1.84 110
-
 Thioacetamide ACV2 mutant Brevibacterium sp.
4.2.1.84 220
-
 adipic acid ACV2 mutant Brevibacterium sp.