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Literature summary extracted from

  • Nagasawa, T.; Takeuchi, K.; Yamada, H.
    Occurrence of a cobalt-induced and cobalt-containing nitrile hydratase in Rhodococcus rhodochrous J1 (1988), Biochem. Biophys. Res. Commun., 155, 1008-1016.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.1.84 synthesis
-
 Rhodococcus rhodochrous
4.2.1.84 synthesis
-
 Pseudomonas chlororaphis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Co2+ 5.7 atoms cobalt/mol enzyme, tightly bound to the protein Rhodococcus rhodochrous
4.2.1.84 Co2+ indispensable for high enzyme activity Rhodococcus rhodochrous
4.2.1.84 Co2+ 0.1% w/v, greatly increases the enzyme activity. Different Co2+ compounds increase the enzyme activity Rhodococcus rhodochrous
4.2.1.84 Co2+ enzyme of J1 strain contains cobalt Rhodococcus rhodochrous
4.2.1.84 Fe2+ at 0.1% w/v does not enhance the enzyme activity Rhodococcus rhodochrous
4.2.1.84 additional information CuSO4, CaCl2, ZnSO4, MnCl2, NaMnO4, FeCl3, FeSO4, AlCl3, BaCl2, SrCl2, NaWO4, SnCl2, BeSO4, NiCl2, PbCl2, LiCl and CoCl2 at 0.1% w/v neither inhibit nor enhance the enzyme activity Rhodococcus rhodochrous

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 26000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
4.2.1.84 29000
-
 x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous
4.2.1.84 505000 530000 sedimentation equilibrium method, gel permeation HPLC Rhodococcus rhodochrous

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Pseudomonas chlororaphis
-
-
-
4.2.1.84 Rhodococcus rhodochrous
-
-
-
4.2.1.84 Rhodococcus rhodochrous J1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84
-
 Rhodococcus rhodochrous
4.2.1.84 ammonium sulfate fractionation, chromatography on DEAE-Sephacel, octyl-Sepharose CL-4B and phenyl-Sepharose CL-4B, 4.91fold purification Rhodococcus rhodochrous

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
-
 Rhodococcus rhodochrous
4.2.1.84 additional information
-
-
 Pseudomonas chlororaphis
4.2.1.84 0.084
-
-
 Rhodococcus rhodochrous
4.2.1.84 5.21
-
 with the addition of 0.1% w/v CoCl2 Rhodococcus rhodochrous

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 29000, alpha, + x * 26000, beta, SDS-PAGE Rhodococcus rhodochrous