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Literature summary extracted from

  • Nagasawa, T.; Ryuno, K.; Yamada, H.
    Nitrile hydratase of Brevibacterium R312 -Purification and characterization- (1986), Biochem. Biophys. Res. Commun., 139, 1305-1312.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.84
-
 Brevibacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 Isobutyronitrile
-
 Brevibacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 3.6
-
 n-valeronitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 8.3
-
 n-butyronitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 9.5
-
 Methacrylonitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 10.5
-
 Propionitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 16.7
-
 acrylonitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 25
-
 Succinonitrile pH 7, 20ºC Brevibacterium sp.
4.2.1.84 30.3
-
 Chloroacetonitrile pH 7, 20ºC Brevibacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.84 Fe2+ contains approximately 3 g-atoms iron/mol enzyme, tightly bound to the protein Brevibacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 26000
-
 x * 26000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 27500
-
 x * 26000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 77000
-
 gel filtration on Sephadex-G150 Brevibacterium sp.
4.2.1.84 85000
-
 sedimentation equilibrium method Brevibacterium sp.
4.2.1.84 87000
-
 gel filtration, HPLC Brevibacterium sp.
4.2.1.84 90000 94000 gel filtration on Cellulofine GCL-2000sf Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Brevibacterium sp.
-
-
-
4.2.1.84 Brevibacterium sp. R312
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 ammonium sulfate fractionation, chromatography on DEAE-Sephacel and phenyl-Sepharose CL-4B and gel filtration, R312 strain, 10.2fold purified Brevibacterium sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
-
 Brevibacterium sp.
4.2.1.84 1890
-
-
 Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acrylonitrile + H2O 78% of the activity with propionitrile Brevibacterium sp. 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 adiponitrile + 2 H2O 108% of the activity with propionitrile Brevibacterium sp. adipic acid amide
-
 ?
4.2.1.84 chloroacetonitrile + H2O 42% of the activity with propionitrile Brevibacterium sp. chloroacetamide
-
 ?
4.2.1.84 chloroacetonitrile + H2O 42% of the activity with propionitrile Brevibacterium sp. R312 chloroacetamide
-
 ?
4.2.1.84 crotononitrile + H2O 19% of the activity with propionitrile Brevibacterium sp. (E)-2-butenoic acid amide
-
 ?
4.2.1.84 ethylene cyanhydrine + H2O
-
 Brevibacterium sp. ?
-
 ?
4.2.1.84 glutaronitrile + H2O 38% of the activity with propionitrile Brevibacterium sp. ?
-
 ?
4.2.1.84 hydroxyacetonitrile + H2O 50% of the activity with propionitrile Brevibacterium sp. hydroxyacetamide
-
 ?
4.2.1.84 malonitrile + H2O 44% of the activity with propionitrile Brevibacterium sp. ?
-
 ?
4.2.1.84 methacrylonitrile + H2O 53% of the activity with propionitrile Brevibacterium sp. methylacrylic acid amide
-
 ?
4.2.1.84 methoxyacetonitrile + H2O
-
 Brevibacterium sp. ?
-
 ?
4.2.1.84 n-butyronitrile + H2O 140% of the activity with propionitrile Brevibacterium sp. n-butyric acid amide
-
 ?
4.2.1.84 n-butyronitrile + H2O 140% of the activity with propionitrile Brevibacterium sp. R312 n-butyric acid amide
-
 ?
4.2.1.84 n-capronitrile + H2O 46% of the activity with propionitrile Brevibacterium sp. n-hexanoic acid amide
-
 ?
4.2.1.84 n-capronitrile + H2O 46% of the activity with propionitrile Brevibacterium sp. R312 n-hexanoic acid amide
-
 ?
4.2.1.84 pivalonitrile + H2O 5% of the activity with propionitrile Brevibacterium sp. 2,2-dimethylpropionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. propionic acid amide i.e. propionamide ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. R312 propionic acid amide i.e. propionamide ?
4.2.1.84 succinonitrile + H2O 135% of the activity with propionitrile Brevibacterium sp. ?
-
 ?
4.2.1.84 valeronitrile + H2O
-
 Brevibacterium sp. n-pentanoic acid amide
-
 ?
4.2.1.84 valeronitrile + H2O
-
 Brevibacterium sp. R312 n-pentanoic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 26000, alpha, + x * 27500, beta, SDS-PAGE Brevibacterium sp.
4.2.1.84 trimer strain R312 Brevibacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 20
-
 assay at Brevibacterium sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.0054
-
 Isobutyronitrile pH 7, 20ºC, competitive inhibitor Brevibacterium sp.