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Literature summary extracted from

  • Hjort, C.M.; Godtfredsen, S.E.; Emborg, C.
    Isolation and characterization of a nitrile hydratase from Rhodococcus sp. (1990), J. Chem. Technol. Biotechnol., 48, 217-226.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.84 synthesis useful for acrylamide production Rhodococcus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 2-mercaptoethanol
-
 Rhodococcus sp.
4.2.1.84 Cu2+ 1-5 mM CuSO4 Rhodococcus sp.
4.2.1.84 EDTA weak Rhodococcus sp.
4.2.1.84 Hg2+ complete inactivation at 0.05 mM Hg2+; HgCl2, 0.01-1 mM Rhodococcus sp.
4.2.1.84 iodoacetic acid
-
 Rhodococcus sp.
4.2.1.84 Propionamide weak inactivation Rhodococcus sp.
4.2.1.84 thiocyanate weak Rhodococcus sp.
4.2.1.84 Zn2+ weak; ZnCl2, 1 mM Rhodococcus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.8
-
 Benzonitrile pH 7, 37°C Rhodococcus sp.
4.2.1.84 6
-
 Propionitrile pH 7, 37°C Rhodococcus sp.
4.2.1.84 14
-
 acrylonitrile pH 7, 37°C Rhodococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 23000
-
 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.
4.2.1.84 26000
-
 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.
4.2.1.84 52000
-
 gel filtration Rhodococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Rhodococcus sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84
-
 Rhodococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 867
-
-
 Rhodococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acetonitrile + H2O
-
 Rhodococcus sp. acetamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Rhodococcus sp. 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 benzonitrile + H2O
-
 Rhodococcus sp. benzoic acid amide
-
 ?
4.2.1.84 cyanide + H2O
-
 Rhodococcus sp. formamide
-
 ?
4.2.1.84 n-butyronitrile + H2O
-
 Rhodococcus sp. n-butyric acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Rhodococcus sp. propionic acid amide
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 dimer 1 * 23000 + 1 * 26000, SDS-PAGE Rhodococcus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 10
-
 stable below Rhodococcus sp.
4.2.1.84 25
-
 5 min, unstable above Rhodococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7.5
-
-
 Rhodococcus sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.84 6 8.3 about 50% of activity maximum at pH 6, about 35% of activity maximum at pH 8.3 Rhodococcus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.84 7 8 highly unstable below pH 7.0 Rhodococcus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.6
-
 Propionamide pH 7, 37°C, mixed-type inhibitor Rhodococcus sp.
4.2.1.84 8.4
-
 thiocyanate pH 7, 37°C, competitive inhibitor Rhodococcus sp.