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Literature summary extracted from

  • Hara, H.; Masai, E.; Katayama, Y.; Fukuda, M.
    The 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in Sphingomonas paucimobilis SYK-6 (2000), J. Bacteriol., 182, 6950-6957.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.83 cysteine
-
 Sphingomonas paucimobilis
4.2.1.83 DTT
-
 Sphingomonas paucimobilis
4.2.1.83 reduced glutathione
-
 Sphingomonas paucimobilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.83 expression in Escherichia coli Sphingomonas paucimobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.83 HgCl2
-
 Sphingomonas paucimobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.83 0.138
-
 (E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid pH 8.0, 30°C Sphingomonas paucimobilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.83 38000
-
 2 * 38000 Sphingomonas paucimobilis
4.2.1.83 69500
-
 gel filtration Sphingomonas paucimobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.83 additional information Sphingomonas paucimobilis enzyme is essential for catabolism of both vanillate and syringate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.83 Sphingomonas paucimobilis Q9KWL6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.83 recombinant enzyme Sphingomonas paucimobilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.83 557
-
-
 Sphingomonas paucimobilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.83 (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O
-
 Sphingomonas paucimobilis 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate
-
 ?
4.2.1.83 additional information enzyme is essential for catabolism of both vanillate and syringate Sphingomonas paucimobilis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.83 homodimer 2 * 38000 Sphingomonas paucimobilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.83 30
-
-
 Sphingomonas paucimobilis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.83 Sphingomonas paucimobilis isoelectric focusing
-
 4.9