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Literature summary extracted from

  • Maruyama, K.
    Purification and properties of gamma-oxalomesaconate hydratase from Pseudomonas ochraceae grown with phthalate (1985), Biochem. Biophys. Res. Commun., 128, 271-277.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.83 2-mercaptoethanol
-
 Pseudomonas straminea
4.2.1.83 cysteine
-
 Pseudomonas straminea
4.2.1.83 DTT
-
 Pseudomonas straminea
4.2.1.83 reduced glutathione
-
 Pseudomonas straminea

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.83 CoCl2
-
 Pseudomonas straminea
4.2.1.83 CuSO4
-
 Pseudomonas straminea
4.2.1.83 HgCl2
-
 Pseudomonas straminea
4.2.1.83 iodoacetamide weak Pseudomonas straminea
4.2.1.83 NEM
-
 Pseudomonas straminea
4.2.1.83 NiCl2
-
 Pseudomonas straminea
4.2.1.83 PCMB
-
 Pseudomonas straminea
4.2.1.83 ZnCl2
-
 Pseudomonas straminea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.83 0.0064
-
 (E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid pH 8.0, 24°C Pseudomonas straminea
4.2.1.83 0.014
-
 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate pH 8.0, 24°C Pseudomonas straminea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.83 additional information no Fe2+- or Mn2+-dependent activation in the presence of DTT under anaerobic conditions Pseudomonas straminea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.83 37000
-
 2 * 37000, SDS-PAGE Pseudomonas straminea
4.2.1.83 68000
-
 gel filtration Pseudomonas straminea

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.83 Pseudomonas straminea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.83
-
 Pseudomonas straminea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.83 108
-
-
 Pseudomonas straminea

Storage Stability

EC Number Storage Stability Organism
4.2.1.83 -20°C, stable for at least 1 month Pseudomonas straminea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.83 (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O i.e. (E)-4-oxobut-1-ene-1,2,4-tricarboxylate, 4-oxalmesaconate, highly specific Pseudomonas straminea 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate r

Subunits

EC Number Subunits Comment Organism
4.2.1.83 homodimer 2 * 37000, SDS-PAGE Pseudomonas straminea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.83 24
-
 assay at Pseudomonas straminea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.83 8.4
-
-
 Pseudomonas straminea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.83 7.2 9.6 50% of activity maximum at pH 7.2 and 9.6 Pseudomonas straminea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.83 Pseudomonas straminea isoelectric focusing
-
 4.9