EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.83 | 2-mercaptoethanol | - |
Pseudomonas straminea | |
4.2.1.83 | cysteine | - |
Pseudomonas straminea | |
4.2.1.83 | DTT | - |
Pseudomonas straminea | |
4.2.1.83 | reduced glutathione | - |
Pseudomonas straminea |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.83 | CoCl2 | - |
Pseudomonas straminea | |
4.2.1.83 | CuSO4 | - |
Pseudomonas straminea | |
4.2.1.83 | HgCl2 | - |
Pseudomonas straminea | |
4.2.1.83 | iodoacetamide | weak | Pseudomonas straminea | |
4.2.1.83 | NEM | - |
Pseudomonas straminea | |
4.2.1.83 | NiCl2 | - |
Pseudomonas straminea | |
4.2.1.83 | PCMB | - |
Pseudomonas straminea | |
4.2.1.83 | ZnCl2 | - |
Pseudomonas straminea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.83 | 0.0064 | - |
(E)-3-Carboxy-5-oxo-2-hex-2-enedioic acid | pH 8.0, 24°C | Pseudomonas straminea | |
4.2.1.83 | 0.014 | - |
2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | pH 8.0, 24°C | Pseudomonas straminea |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.1.83 | additional information | no Fe2+- or Mn2+-dependent activation in the presence of DTT under anaerobic conditions | Pseudomonas straminea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.2.1.83 | 37000 | - |
2 * 37000, SDS-PAGE | Pseudomonas straminea |
4.2.1.83 | 68000 | - |
gel filtration | Pseudomonas straminea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.83 | Pseudomonas straminea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.83 | - |
Pseudomonas straminea |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.83 | 108 | - |
- |
Pseudomonas straminea |
EC Number | Storage Stability | Organism |
---|---|---|
4.2.1.83 | -20°C, stable for at least 1 month | Pseudomonas straminea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.83 | (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O | i.e. (E)-4-oxobut-1-ene-1,2,4-tricarboxylate, 4-oxalmesaconate, highly specific | Pseudomonas straminea | 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate | i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate | r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.1.83 | homodimer | 2 * 37000, SDS-PAGE | Pseudomonas straminea |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.83 | 24 | - |
assay at | Pseudomonas straminea |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.83 | 8.4 | - |
- |
Pseudomonas straminea |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.83 | 7.2 | 9.6 | 50% of activity maximum at pH 7.2 and 9.6 | Pseudomonas straminea |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.1.83 | Pseudomonas straminea | isoelectric focusing | - |
4.9 |