BRENDA - Enzyme Database

Characterization of two recombinant Drosophila calpains. CALPA and a novel homolog, CALPB

Jekely, G.; Friedrich, P.; J. Biol. Chem. 274, 23893-23900 (1999)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.22.B36
phosphatidic acid
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol 4,5-bisphosphate
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol 4-monophosphate
activates
Drosophila melanogaster
3.4.22.B37
additional information
no activation by phosphatidylinositol 4,5-diphosphate, phosphatidylinositol 4-monophosphate, phosphatidylinositol or phosphatidic acid. N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation
Drosophila melanogaster
Cloned(Commentary)
EC Number
Commentary
Organism
3.4.22.B36
expression of wild-type and truncated enzyme form, lacking the CALPA-specific unique insertion region
Drosophila melanogaster
3.4.22.B37
expression in Escherichia coli
Drosophila melanogaster
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.4.22.B36
additional information
the truncated enzyme form lacking the CALPA-specific unique insertion region. Although it lacks the 16-amino acid long putative membrane-anchoring segment, its activation by phospholipids is similar to that of the full-length CALPA protein
Drosophila melanogaster
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.B37
cytoplasm
in Schneider S2 cells, with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes
Drosophila melanogaster
5737
-
3.4.22.B37
membrane
with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes
Drosophila melanogaster
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.22.B36
Ca2+
activates, half-maximal activation of the full-length enzyme at 2.18 mM and for the truncated enzyme at 3.23 mM
Drosophila melanogaster
3.4.22.B36
Mn2+
slight activation
Drosophila melanogaster
3.4.22.B37
Ca2+
half-maximal activation at 3.28 mM for the soluble calpain B and 4.38 mM for the renatured calpain B
Drosophila melanogaster
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.B37
90134
-
x * 90134, calculation from nucleotide sequence
Drosophila melanogaster
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.4.22.B36
Drosophila melanogaster
-
-
-
3.4.22.B37
Drosophila melanogaster
-
-
-
3.4.22.B37
Drosophila melanogaster
Q9VT65
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.4.22.B37
-
Drosophila melanogaster
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.B36
casein + H2O
-
648418
Drosophila melanogaster
hydrolyzed casein
-
-
-
?
3.4.22.B36
additional information
the enzyme undergoes N-terminal autolysis in a Ca2+-dependent manner
648418
Drosophila melanogaster
?
-
-
-
-
3.4.22.B37
additional information
N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation
648418
Drosophila melanogaster
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.4.22.B37
?
x * 90134, calculation from nucleotide sequence
Drosophila melanogaster
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.4.22.B36
phosphatidic acid
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol 4,5-bisphosphate
activates
Drosophila melanogaster
3.4.22.B36
phosphatidylinositol 4-monophosphate
activates
Drosophila melanogaster
3.4.22.B37
additional information
no activation by phosphatidylinositol 4,5-diphosphate, phosphatidylinositol 4-monophosphate, phosphatidylinositol or phosphatidic acid. N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation
Drosophila melanogaster
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.B36
expression of wild-type and truncated enzyme form, lacking the CALPA-specific unique insertion region
Drosophila melanogaster
3.4.22.B37
expression in Escherichia coli
Drosophila melanogaster
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.4.22.B36
additional information
the truncated enzyme form lacking the CALPA-specific unique insertion region. Although it lacks the 16-amino acid long putative membrane-anchoring segment, its activation by phospholipids is similar to that of the full-length CALPA protein
Drosophila melanogaster
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.4.22.B37
cytoplasm
in Schneider S2 cells, with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes
Drosophila melanogaster
5737
-
3.4.22.B37
membrane
with increasing Ca2+ concentrations the enzyme adheres to intracellular membranes
Drosophila melanogaster
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.22.B36
Ca2+
activates, half-maximal activation of the full-length enzyme at 2.18 mM and for the truncated enzyme at 3.23 mM
Drosophila melanogaster
3.4.22.B36
Mn2+
slight activation
Drosophila melanogaster
3.4.22.B37
Ca2+
half-maximal activation at 3.28 mM for the soluble calpain B and 4.38 mM for the renatured calpain B
Drosophila melanogaster
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.22.B37
90134
-
x * 90134, calculation from nucleotide sequence
Drosophila melanogaster
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.22.B37
-
Drosophila melanogaster
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.4.22.B36
casein + H2O
-
648418
Drosophila melanogaster
hydrolyzed casein
-
-
-
?
3.4.22.B36
additional information
the enzyme undergoes N-terminal autolysis in a Ca2+-dependent manner
648418
Drosophila melanogaster
?
-
-
-
-
3.4.22.B37
additional information
N-terminal autolysis in a Ca2+-dependent manner which runs parallel with the enzyme activation
648418
Drosophila melanogaster
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.22.B37
?
x * 90134, calculation from nucleotide sequence
Drosophila melanogaster