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Literature summary extracted from

  • Rode, H.; Giffhorn, F.
    D-(-)-tartrate dehydratase of Rhodopseudomonas sphaeroides: purification, characterization, and application to enzymatic determination of D-(-)-tartrate (1982), J. Bacteriol., 150, 1061-1068.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.81
-
 Cereibacter sphaeroides

General Stability

EC Number General Stability Organism
4.2.1.81 omission of Mg2+ from standard buffer results in rapid inactivation during purification with irreversible loss of activity after ammonium sulfate precipitation, Mg2+, Mn2+, Zn2+, Ca2+ or Co2+ stabilizes, not Ni2+ Cereibacter sphaeroides

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.81 beta-mercaptoethanol
-
 Cereibacter sphaeroides
4.2.1.81 citrate weak Cereibacter sphaeroides
4.2.1.81 Cu2+
-
 Cereibacter sphaeroides
4.2.1.81 D-malate weak Cereibacter sphaeroides
4.2.1.81 dithioerythritol
-
 Cereibacter sphaeroides
4.2.1.81 EDTA strong Cereibacter sphaeroides
4.2.1.81 Fe2+
-
 Cereibacter sphaeroides
4.2.1.81 glycolate weak Cereibacter sphaeroides
4.2.1.81 L(+)-Tartrate weak Cereibacter sphaeroides
4.2.1.81 L-malate weak Cereibacter sphaeroides
4.2.1.81 meso-tartrate
-
 Cereibacter sphaeroides
4.2.1.81 additional information no inhibition by iodoacetamide, N-ethylmaleimide; no inhibition by iodoacetate Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.81 0.18
-
 (S,S)-Tartrate
-
 Cereibacter sphaeroides

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.81 cytosol
-
 Cereibacter sphaeroides 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.81 divalent cations not Cereibacter sphaeroides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.81 39500
-
 4 * 39500, SDS-PAGE Cereibacter sphaeroides
4.2.1.81 158000
-
 gel filtration, ultracentrifugation Cereibacter sphaeroides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.81 (S,S)-tartrate Cereibacter sphaeroides
-
 oxaloacetate + H2O
-
 ir
4.2.1.81 (S,S)-tartrate Cereibacter sphaeroides Si4 / DSM 8371
-
 oxaloacetate + H2O
-
 ir
4.2.1.81 additional information Cereibacter sphaeroides involved in D-tartrate catabolism ?
-
 ?
4.2.1.81 additional information Cereibacter sphaeroides Si4 / DSM 8371 involved in D-tartrate catabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.81 Cereibacter sphaeroides
-
-
-
4.2.1.81 Cereibacter sphaeroides Si4 / DSM 8371
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.81
-
 Cereibacter sphaeroides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.81 7.2
-
-
 Cereibacter sphaeroides

Storage Stability

EC Number Storage Stability Organism
4.2.1.81 -20°C, 1.5 mg enzyme/ml 0.1 M Tris-HCl buffer, pH 7.2, 1 mM MgCl2, 20% loss of activity within 5 months Cereibacter sphaeroides
4.2.1.81 22°C, 50% loss of activity within 10 days Cereibacter sphaeroides
4.2.1.81 4°C, 50% loss of activity within 6 weeks Cereibacter sphaeroides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.81 (S,S)-tartrate
-
 Cereibacter sphaeroides oxaloacetate + H2O
-
 ir
4.2.1.81 (S,S)-tartrate no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol Cereibacter sphaeroides oxaloacetate + H2O at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate ir
4.2.1.81 (S,S)-tartrate
-
 Cereibacter sphaeroides Si4 / DSM 8371 oxaloacetate + H2O
-
 ir
4.2.1.81 (S,S)-tartrate no substrates are L(+)-tartrate, meso-tartrate, glyceraldehyde, glycerate, glycerol, gluconate, 6-phosphogluconate, galactonate, mannonate, mannitol or sorbitol Cereibacter sphaeroides Si4 / DSM 8371 oxaloacetate + H2O at high or low pH-values or in the presence of metal ions spontaneous decarboxylation to pyruvate ir
4.2.1.81 additional information involved in D-tartrate catabolism Cereibacter sphaeroides ?
-
 ?
4.2.1.81 additional information involved in D-tartrate catabolism Cereibacter sphaeroides Si4 / DSM 8371 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.81 tetramer 4 * 39500, SDS-PAGE Cereibacter sphaeroides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.81 30
-
 assay at Cereibacter sphaeroides
4.2.1.81 48
-
-
 Cereibacter sphaeroides

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.81 30
-
 stable at least 5 min at pH 6-8.7, 50% loss of activity at pH 4.9 and pH 9.4 after 5 min Cereibacter sphaeroides
4.2.1.81 45
-
 30 min, 50% loss of activity Cereibacter sphaeroides
4.2.1.81 50
-
 3 min, 50% loss of activity Cereibacter sphaeroides
4.2.1.81 55
-
 1 min, 50% loss of activity Cereibacter sphaeroides
4.2.1.81 60
-
 12 s, 50% loss of activity Cereibacter sphaeroides

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.81 19.8
-
 D-Tartrate
-
 Cereibacter sphaeroides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.81 additional information
-
 highest activity in Tris-HCl buffer, less effective are N-2'-hydroxyethylpiperazine-2-ethanesulfonic acid, glycylglycine, triethanolamine-HCl, N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid, imidazole or potassium phosphate buffer Cereibacter sphaeroides
4.2.1.81 6.2 7.2
-
 Cereibacter sphaeroides

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.81 4.9
-
 5 min, 50% loss of activity at 30°C Cereibacter sphaeroides
4.2.1.81 6 8.7 stable at least 5 min at 30°C Cereibacter sphaeroides
4.2.1.81 9.4
-
 5 min, 50% loss of activity at 30°C Cereibacter sphaeroides

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.81 Cereibacter sphaeroides isoelectric focusing
-
 5.5