Literature summary extracted from

Pollard, J.R.; Bugg, T.D.H.
Purification, characterization and reaction mechanism of monofunctional 2-hydroxypentadienoic acid hydratase from Escherichia coli (1998), Eur. J. Biochem., 251, 98-106.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.2.1.80	expression in Escherichia coli	Escherichia coli

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
4.2.1.80	EDTA	-	Escherichia coli
4.2.1.80	sodium oxalate	-	Escherichia coli

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.2.1.80	0.041	-	2-hydroxypentadienoic acid	pH 6	Escherichia coli

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.2.1.80	Mn2+	reqirement of divalent cations	Escherichia coli

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.2.1.80	28000	-	gel filtration	Escherichia coli

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.2.1.80	Escherichia coli	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.2.1.80	-	Escherichia coli

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
4.2.1.80	538	-	MonoQ pool	Escherichia coli

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.2.1.80	2-hydroxypentadienoic acid	-	Escherichia coli	?	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
4.2.1.80	2-hydroxypentadienoic acid hydratase	-	Escherichia coli

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
4.2.1.80	0.0049	-	sodium oxalate	pH 6	Escherichia coli

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Release 2023.1 (January 2023)