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Literature summary extracted from

  • Yamada, E.W; Jakoby, W.B.
    Enzymatic utilization of acetylenic compounds. I. An enzyme converting acetylenedicarboxylic acid to pyruvate (1958), J. Biol. Chem., 233, 706-711.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.1.78 loss of stabililty by freezing and thawing Pseudomonas sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.78 Al(NO3)3
-
Pseudomonas sp.
4.1.1.78 BaCl2
-
Pseudomonas sp.
4.1.1.78 CN-
-
Pseudomonas sp.
4.1.1.78 CoCl2
-
Pseudomonas sp.
4.1.1.78 EDTA
-
Pseudomonas sp.
4.1.1.78 Fe(NO3)3
-
Pseudomonas sp.
4.1.1.78 HgCl2
-
Pseudomonas sp.
4.1.1.78 NiCl2
-
Pseudomonas sp.
4.1.1.78 p-chloromercuribenzoate
-
Pseudomonas sp.
4.1.1.78 ZnSO4
-
Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.78 0.07
-
acetylene dicarboxylate 23°C, pH 7.3 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.78 acetylene dicarboxylate Pseudomonas sp.
-
pyruvate + CO2
-
ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.78 no activity in Clostridium cylindrosporum
-
-
-
4.1.1.78 no activity in Clostridium kluyveri
-
-
-
4.1.1.78 no activity in Escherichia coli
-
-
-
4.1.1.78 no activity in Pseudomonas fluorescens
-
-
-
4.1.1.78 no activity in Rattus norvegicus
-
-
-
4.1.1.78 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.78
-
Pseudomonas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.78 cell culture
-
Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.78 463
-
-
Pseudomonas sp.

Storage Stability

EC Number Storage Stability Organism
4.1.1.78 -20°C, 0.005 mM 2-mercaptoethanol, 10 days, less than 10% loss of activity Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.78 acetylene dicarboxylate
-
Pseudomonas sp. pyruvate + CO2
-
ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.78 23
-
enzyme assay at Pseudomonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.78 20
-
-
Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.78 7.3 7.8
-
Pseudomonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.78 6.5 8.5
-
Pseudomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.78 7.5
-
highest stability at 3°C for 17 h Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.78 additional information no requirement Pseudomonas sp.