EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.72 | 0.001 | - |
L-3-Methyl-2-oxopentanoate | and 2-oxoisopentanoate, 2-oxoisohexanoate, value below, 30°C, pH 7.5 | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.72 | ferricyanide | stimulates | Bacillus subtilis | |
4.1.1.72 | additional information | no requirement for MgCl2 | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 33000 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
4.1.1.72 | 39800 | - |
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
4.1.1.72 | 175000 | - |
gel filtration | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.72 | 3-methyl-2-oxopentanoate | Bacillus subtilis | - |
CO2 + 2-methylbutanal | - |
? | |
4.1.1.72 | additional information | Bacillus subtilis | essential for the synthesis of branched-chain fatty acids | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.72 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.72 | - |
Bacillus subtilis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 0.02 | - |
30°C, pH 7.5 | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.72 | 2-oxoglutarate | 5% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + succinate semialdehyde | - |
? | |
4.1.1.72 | 2-oxoisohexanoate | 38% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | CO2 + isopentanal | - |
? | |
4.1.1.72 | 2-oxoisopentanoate | 63% of the activity with L-3-methyl- | Bacillus subtilis | CO2 + isobutanal | - |
? | |
4.1.1.72 | 3-methyl-2-oxopentanoate | - |
Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
4.1.1.72 | L-3-methyl-2-oxopentanoate | stereospecificity towards the L-isomer | Bacillus subtilis | CO2 + 2-methylbutanal | - |
? | |
4.1.1.72 | additional information | essential for the synthesis of branched-chain fatty acids | Bacillus subtilis | ? | - |
? | |
4.1.1.72 | pyruvate | 25% of the activity with L-3-methyl-2-oxopentanoate | Bacillus subtilis | ethanal + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.72 | tetramer | 2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE | Bacillus subtilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 30 | - |
- |
Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 7.5 | - |
- |
Bacillus subtilis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.72 | 6 | 9 | pH 6: about 60% of maximum activity, pH 9: about 55% of maximum activity | Bacillus subtilis |