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Literature summary extracted from

  • Oku, H.; Kaneda, T.
    Biosynthesis of branched-chain fatty acids in Bacillus subtilis. A decarboxylase is essential for branched-chain fatty acid synthetase (1988), J. Biol. Chem., 263, 18386-18396.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.72 0.001
-
L-3-Methyl-2-oxopentanoate and 2-oxoisopentanoate, 2-oxoisohexanoate, value below, 30°C, pH 7.5 Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.72 ferricyanide stimulates Bacillus subtilis
4.1.1.72 additional information no requirement for MgCl2 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.72 33000
-
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE   Bacillus subtilis
4.1.1.72 39800
-
2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE   Bacillus subtilis
4.1.1.72 175000
-
gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.72 3-methyl-2-oxopentanoate Bacillus subtilis
-
CO2 + 2-methylbutanal
-
?
4.1.1.72 additional information Bacillus subtilis essential for the synthesis of branched-chain fatty acids ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.72 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.72
-
Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.72 0.02
-
30°C, pH 7.5 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.72 2-oxoglutarate 5% of the activity with L-3-methyl-2-oxopentanoate Bacillus subtilis CO2 + succinate semialdehyde
-
?
4.1.1.72 2-oxoisohexanoate 38% of the activity with L-3-methyl-2-oxopentanoate Bacillus subtilis CO2 + isopentanal
-
?
4.1.1.72 2-oxoisopentanoate 63% of the activity with L-3-methyl- Bacillus subtilis CO2 + isobutanal
-
?
4.1.1.72 3-methyl-2-oxopentanoate
-
Bacillus subtilis CO2 + 2-methylbutanal
-
?
4.1.1.72 L-3-methyl-2-oxopentanoate stereospecificity towards the L-isomer Bacillus subtilis CO2 + 2-methylbutanal
-
?
4.1.1.72 additional information essential for the synthesis of branched-chain fatty acids Bacillus subtilis ?
-
?
4.1.1.72 pyruvate 25% of the activity with L-3-methyl-2-oxopentanoate Bacillus subtilis ethanal + CO2
-
?

Subunits

EC Number Subunits Comment Organism
4.1.1.72 tetramer 2 * 33000, A-subunit, + 2 * 39800, B-subunit, SDS-PAGE   Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.72 30
-
-
Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.72 7.5
-
-
Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.72 6 9 pH 6: about 60% of maximum activity, pH 9: about 55% of maximum activity Bacillus subtilis