EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.71 | 2-mercaptoethanol | stimulates | Euglena gracilis | |
4.1.1.71 | DTT | stimulates | Euglena gracilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.71 | 2-oxoglutarate | above 2 mM substrate inhibition | Euglena gracilis | |
4.1.1.71 | Cu2+ | - |
Euglena gracilis | |
4.1.1.71 | Hg2+ | - |
Euglena gracilis | |
4.1.1.71 | NEM | - |
Euglena gracilis | |
4.1.1.71 | Ni2+ | - |
Euglena gracilis | |
4.1.1.71 | PCMB | - |
Euglena gracilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.71 | 0.33 | - |
2-oxoglutarate | pH 7.0, 30°C | Euglena gracilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.1.1.71 | mitochondrion | exclusively | Euglena gracilis | 5739 | - |
4.1.1.71 | additional information | not: cytosol, microsomes | Euglena gracilis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.71 | Ca2+ | can partially replace Mg2+ in activation | Euglena gracilis | |
4.1.1.71 | Co2+ | can partially replace Mg2+ in activation | Euglena gracilis | |
4.1.1.71 | MgCl2 | required | Euglena gracilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.71 | additional information | Euglena gracilis | the enzyme has a physiologically important role coupling with succinate-semialdehyde dehydrogenase in the tricarboxylic acid cycle | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.71 | Euglena gracilis | - |
lacking a 2-oxoglutarate dehydrogenase complex (EC 1.2.4.2 + EC 2.3.1.61 + EC 1.8.1.4) | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.71 | 2-oxoglutarate | highly specific | Euglena gracilis | succinate semialdehyde + CO2 | - |
? | |
4.1.1.71 | additional information | the enzyme has a physiologically important role coupling with succinate-semialdehyde dehydrogenase in the tricarboxylic acid cycle | Euglena gracilis | ? | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.71 | 40 | - |
- |
Euglena gracilis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.71 | 35 | 40 | 35-40°C: activity maximum, maximum activity is maintained up to 60°C | Euglena gracilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.71 | 70 | - |
complete loss of activity at 70°C | Euglena gracilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.71 | 7 | - |
- |
Euglena gracilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.71 | NADP+ | stimulates | Euglena gracilis | |
4.1.1.71 | thiamine diphosphate | required | Euglena gracilis | |
4.1.1.71 | thiamine diphosphate | Km: 0.056 mM | Euglena gracilis |