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Literature summary extracted from

  • Braune, A.; Bendrat, K.; Rospert, S.; Buckel, W.
    The sodium ion translocating glutaconyl-CoA decarboxylase from Acidaminococcus fermentans: cloning and function of the genes forming a second operon (1999), Mol. Microbiol., 31, 473-487.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.4.1.4 decarboxylase operon cloned and sequenced, overproduced in Escherichia coli Acidaminococcus fermentans
7.2.4.5 four different subunits Acidaminococcus fermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.4.1.4 11624
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 14000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 14133
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 25000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 36000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 38902
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 64300
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
6.4.1.4 65000
-
 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
7.2.4.5 12000
-
 x * 65000, alpha, carboxytransferase, + x * 39000, beta, decarboxylase, + x * 14000, gamma, biotin carrier, + x * 12000, delta, membrane anchor, deduced from gene sequence, expression and purification of alpha leads to a homotrimer, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 14000
-
 x * 65000, alpha, carboxytransferase, + x * 39000, beta, decarboxylase, + x * 14000, gamma, biotin carrier, + x * 12000, delta, membrane anchor, deduced from gene sequence, expression and purification of alpha leads to a homotrimer, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 39000
-
 x * 65000, alpha, carboxytransferase, + x * 39000, beta, decarboxylase, + x * 14000, gamma, biotin carrier, + x * 12000, delta, membrane anchor, deduced from gene sequence, expression and purification of alpha leads to a homotrimer, SDS-PAGE Acidaminococcus fermentans
7.2.4.5 65000
-
 x * 65000, alpha, carboxytransferase, + x * 39000, beta, decarboxylase, + x * 14000, gamma, biotin carrier, + x * 12000, delta, membrane anchor, deduced from gene sequence, expression and purification of alpha leads to a homotrimer, SDS-PAGE Acidaminococcus fermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- Acidaminococcus fermentans key enzyme in the fermentation of glutamate to ammonia, carbon dioxide, hydrogen, acetate and butyrate ADP + phosphate + 3-methylglutaconyl-CoA
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.4.1.4 Acidaminococcus fermentans
-
-
-
7.2.4.5 Acidaminococcus fermentans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.4.1.4 recombinant alpha-subunit Acidaminococcus fermentans

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3-
-
 Acidaminococcus fermentans ADP + phosphate + 3-methylglutaconyl-CoA
-
 r
6.4.1.4 ATP + 3-methylcrotonyl-CoA + HCO3- key enzyme in the fermentation of glutamate to ammonia, carbon dioxide, hydrogen, acetate and butyrate Acidaminococcus fermentans ADP + phosphate + 3-methylglutaconyl-CoA
-
 r
6.4.1.4 glutaconyl-CoA + H+
-
 Acidaminococcus fermentans crotonyl-CoA + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
6.4.1.4 tetramer 1 * 65000 + 1 * 36000 + 1 * 25000 + 1 * 14000, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, SDS-PAGE, 1 * 64300 + 1 * 38902 + 1 * 14133 + 1 * 11624, alpha GcdA, beta GcdB, gamma GcdC, delta GcdD, amino acid sequence Acidaminococcus fermentans
7.2.4.5 ? x * 65000, alpha, carboxytransferase, + x * 39000, beta, decarboxylase, + x * 14000, gamma, biotin carrier, + x * 12000, delta, membrane anchor, deduced from gene sequence, expression and purification of alpha leads to a homotrimer, SDS-PAGE Acidaminococcus fermentans

Synonyms

EC Number Synonyms Comment Organism
6.4.1.4 glutaconyl-CoA decarboxylase
-
 Acidaminococcus fermentans
6.4.1.4 glutaconyl-CoA:biotin carboxytransferase
-
 Acidaminococcus fermentans