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Literature summary extracted from

  • Hohn, T.M.; Vanmiddlesworth, F.
    Purification and characterization of the sesquiterpene cyclase trichodiene synthetase from Fusarium sporotrichioides (1986), Arch. Biochem. Biophys., 251, 756-761.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.6 farnesyl diphosphate analogs
-
 Fusarium sporotrichioides
4.2.3.6 Mn2+ 0.01 mM, can partially replace Mg2+, inhibition at higher concentration Fusarium sporotrichioides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.6 0.000065
-
 farnesyl diphosphate 30°C, pH 7.5 Fusarium sporotrichioides

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.6 Mg2+ required, optimum concentration: 1.0 mM, Km: 0.1 mM Fusarium sporotrichioides
4.2.3.6 Mn2+ at 0.01 mM, can partially replace Mg2+, inhibition at higher concentration Fusarium sporotrichioides

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.6 45000
-
 2 * 45000, SDS-PAGE Fusarium sporotrichioides
4.2.3.6 80000
-
 gel filtration Fusarium sporotrichioides

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.6 (E,E)-farnesyl diphosphate Fusarium sporotrichioides
-
 trichodiene + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.6 Fusarium sporotrichioides
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.6
-
 Fusarium sporotrichioides

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.6 additional information
-
-
 Fusarium sporotrichioides

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.6 (E,E)-farnesyl diphosphate
-
 Fusarium sporotrichioides trichodiene + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.6 dimer 2 * 45000, SDS-PAGE Fusarium sporotrichioides

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.3.6 30
-
 assay at Fusarium sporotrichioides

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.6 6.8 7.8
-
 Fusarium sporotrichioides

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.6 6 8 pH 6.0: about 40% of activity maximum, pH 8.0: about 70% of activity maximum Fusarium sporotrichioides