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Literature summary extracted from

  • Barth, P.T.; Bolton, L.; Thomson, J.C.
    Cloning and partial sequencing of an operon encoding two Pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity (1992), J. Bacteriol., 174, 2612-2619.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.8.1.2 synthesis production of (S)-2-chloropropionic acid, which is used in the synthesis of optically active phenoxypropionic acid herbicides Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli and Pseudomonas putida hosts by using broad-host-range vectors Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.8.1.2 cytoplasm
-
 Pseudomonas putida 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 33600
-
 calculated from amino acid sequence Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas putida
-
-
-
3.8.1.2 Pseudomonas putida
-
 AJ1
-
3.8.1.2 Pseudomonas putida AJ1
-
 AJ1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (R,S)-2-chloropropionic acid + H2O
-
 Pseudomonas putida (S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
 ?
3.8.1.2 (R,S)-2-chloropropionic acid + H2O
-
 Pseudomonas putida AJ1 (S)-lactic acid + (S)-2-chloropropionic acid + HCl
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 30
-
-
 Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 7.4
-
-
 Pseudomonas putida