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Literature summary extracted from

  • Pang, B.C.M.; Tsang, J.S.H.
    Mutagenic analysis of the conserved residues in dehalogenase IVa of Burkholderia cepacia MBA4 (2001), FEMS Microbiol. Lett., 204, 135-140.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.2 D11E totally inactive in catalysis Burkholderia cepacia
3.8.1.2 D11N totally inactive in catalysis Burkholderia cepacia
3.8.1.2 D11S totally inactive in catalysis Burkholderia cepacia
3.8.1.2 D181N totally inactive in catalysis Burkholderia cepacia
3.8.1.2 additional information point mutations on 29 conserved charged and/or polar residues are generated and characterized Burkholderia cepacia
3.8.1.2 N178D defective in catalysis Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Burkholderia cepacia
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Burkholderia cepacia MBA4
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Burkholderia cepacia
-
 MBA4
-
3.8.1.2 Burkholderia cepacia MBA4
-
 MBA4
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Burkholderia cepacia (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Burkholderia cepacia MBA4 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Burkholderia cepacia D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Burkholderia cepacia MBA4 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Burkholderia cepacia glycolate + HCl
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Burkholderia cepacia MBA4 glycolate + HCl
-
 ?