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Literature summary extracted from

  • Jones, D.H.A.; Barth, P.T.; Byrom, D.; Thomas, C.M.
    Nucleotide sequence of the structural gene encoding a 2-haloalkanoic acid dehalogenase of Pseudomonas putida strain AJ1 and purification of the encoded protein (1992), J. Gen. Microbiol., 138, 675-683.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 25687
-
 1 * 26000, SDS-PAGE, 1 * 25687, calculated from amino acid sequence Pseudomonas putida
3.8.1.2 26000
-
 1 * 26000, SDS-PAGE, 1 * 25687, calculated from amino acid sequence Pseudomonas putida
3.8.1.2 79000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Pseudomonas putida
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Pseudomonas putida
-
-
-
3.8.1.2 Pseudomonas putida Q52087 AJ1
-
3.8.1.2 Pseudomonas putida AJ1 Q52087 AJ1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas putida (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Pseudomonas putida AJ1 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2,3-dibromopropionate + H2O
-
 Pseudomonas putida ?
-
 ?
3.8.1.2 2,3-dibromopropionate + H2O
-
 Pseudomonas putida AJ1 ?
-
 ?
3.8.1.2 2,3-dichloropropionate + H2O
-
 Pseudomonas putida ?
-
 ?
3.8.1.2 2,3-dichloropropionate + H2O
-
 Pseudomonas putida AJ1 ?
-
 ?
3.8.1.2 2-monobromobutyrate + H2O
-
 Pseudomonas putida ?
-
 ?
3.8.1.2 2-monochlorobutyrate + H2O
-
 Pseudomonas putida ?
-
 ?
3.8.1.2 2-monochlorobutyrate + H2O
-
 Pseudomonas putida AJ1 ?
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas putida D-lactate + HCl
-
 ?
3.8.1.2 L-2-chloropropionate + H2O
-
 Pseudomonas putida AJ1 D-lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Pseudomonas putida D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Pseudomonas putida glycolate + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 tetramer 1 * 26000, SDS-PAGE, 1 * 25687, calculated from amino acid sequence Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 10 11
-
 Pseudomonas putida

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.2 Pseudomonas putida
-
-
 5