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Literature summary extracted from

  • Köhler, R.; Brokamp, A.; Schwarze, R.; Reiting, R.H.; Schmidt, F.R.J.
    Characteristics and DNA-sequence of a cryptic haloalkanoic acid dehalogenase from Agrobacterium tumefaciens RS5 (1998), Curr. Microbiol., 36, 96-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.8.1.2 expression in Escherichia coli Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 thiol reagents only when recombinant in E.coli Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 2,2-dichloropropionic acid + H2O Agrobacterium tumefaciens
-
 ?
-
 ?
3.8.1.2 2-chloropropionate + H2O Agrobacterium tumefaciens
-
 lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O Agrobacterium tumefaciens RS5
-
 lactate + HCl
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Agrobacterium tumefaciens
-
 cryptic gene
-
3.8.1.2 Agrobacterium tumefaciens RS5
-
 cryptic gene
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 0.23
-
-
 Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 2,2-dichloropropionic acid + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Agrobacterium tumefaciens lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Agrobacterium tumefaciens RS5 lactate + HCl
-
 ?
3.8.1.2 dichloroacetic acid + H2O
-
 Agrobacterium tumefaciens ?
-
 ?
3.8.1.2 dichloroacetic acid + H2O
-
 Agrobacterium tumefaciens RS5 ?
-
 ?
3.8.1.2 L-2-chloropropionic acid + H2O
-
 Agrobacterium tumefaciens D-2-hydroxypropionate + HCl
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Agrobacterium tumefaciens glycolate + HBr
-
 ?
3.8.1.2 monobromoacetate + H2O
-
 Agrobacterium tumefaciens RS5 glycolate + HBr
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Agrobacterium tumefaciens glycolate + HCl
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Agrobacterium tumefaciens RS5 glycolate + HCl
-
 ?
3.8.1.2 monoiodoacetate + H2O
-
 Agrobacterium tumefaciens glycolate + HI
-
 ?
3.8.1.2 monoiodoacetate + H2O
-
 Agrobacterium tumefaciens RS5 glycolate + HI
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 65
-
-
 Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 60
-
 recombinant in Escherichia coli Agrobacterium tumefaciens