EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.8.1.2 | additional information | mutant library with reduced activity | Burkholderia cepacia |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.2 | additional information | - |
additional information | comparison of KM of wild-type and mutant enzymes | Burkholderia cepacia |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.8.1.2 | Burkholderia cepacia | - |
i.e. Pseudomonas cepacia MBA4 | - |
3.8.1.2 | Burkholderia cepacia MBA4 | - |
i.e. Pseudomonas cepacia MBA4 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.8.1.2 | - |
Burkholderia cepacia |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.8.1.2 | 2-chloropropionate + H2O | - |
Burkholderia cepacia | lactate + HCl | - |
? | |
3.8.1.2 | 2-chloropropionate + H2O | - |
Burkholderia cepacia MBA4 | lactate + HCl | - |
? | |
3.8.1.2 | monochloroacetate + H2O | - |
Burkholderia cepacia | glycolate + HCl | - |
? | |
3.8.1.2 | monochloroacetate + H2O | - |
Burkholderia cepacia MBA4 | glycolate + HCl | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.8.1.2 | additional information | - |
additional information | comparison of kcat of wild-type and mutant enzymes | Burkholderia cepacia |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.8.1.2 | Burkholderia cepacia | the mutation from an acidic to a basic amino acid leads to an alteration in the enzyme pI valiue from 5.1 to 6.0 | - |
5.1 |