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Literature summary extracted from

  • Diez, A.; Prieto, M.I.; Alvarez, M.J.; Bautista, J.M.; Garrido, A.; Puyet, A.
    Improved catalytic performance of a 2-haloacid dehalogenase from Azotobacter sp. by ion-exchange immobilisation (1996), Biochem. Biophys. Res. Commun., 220, 828-833.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.8.1.2 environmental protection detoxification of halogenated herbicides, solvents and other xenobiotic compounds by immobilized enzyme Azotobacter sp.
3.8.1.2 synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Azotobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.1
-
 Monochloroacetate pH 8.0, 30°C Azotobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 L-2-haloalkanoic acid + H2O Azotobacter sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Azotobacter sp. RC26
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Azotobacter sp.
-
-
-
3.8.1.2 Azotobacter sp. RC26
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Azotobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Azotobacter sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Azotobacter sp. RC26 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. RC26 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Azotobacter sp. glycolate + HCl
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Azotobacter sp. RC26 glycolate + HCl
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.2 20 60
-
 Azotobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 additional information
-
 activity and stability at different temperatures Azotobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.8.1.2 8 10
-
 Azotobacter sp.