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Literature summary extracted from

  • Diez, A.; Prieto, M.I.; Alvarez, M.J.; Bautista, J.M.; Puyet, A.; Garrido-Pertierra, A.
    Purification and properties of a high-affinity L-2-haloacid dehalogenase from Azotobacter sp. strain RC26 (1996), Lett. Appl. Microbiol., 23, 279-282.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.8.1.2 synthesis production of optically active 2-hydroxyalkanoic acids and 2-haloalkanoic acids for chiral synthesis in industry Azotobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.2 Cu2+
-
 Azotobacter sp.
3.8.1.2 FeSO4 above 10 mM Azotobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.2 0.1
-
 Monochloroacetate pH 8.0, 30°C Azotobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.2 28000
-
 gel filtration Azotobacter sp.
3.8.1.2 29000
-
 1 * 29000 SDS-PAGE Azotobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.2 2-chloropropionate + H2O Azotobacter sp.
-
 lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O Azotobacter sp. RC26
-
 lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Azotobacter sp.
-
 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O Azotobacter sp. RC26
-
 D-2-hydroxyalkanoic acid + halide
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.8.1.2 Acetone 100% activity at 10 mM Azotobacter sp.
3.8.1.2 chloroform 100% activity at 10 mM Azotobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.2 Azotobacter sp.
-
-
-
3.8.1.2 Azotobacter sp. RC26
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.8.1.2
-
 Azotobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.8.1.2 0.7
-
 coupled assay with lactate dehydrogenase Azotobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.2 (S)-2-haloacid + H2O
-
 Azotobacter sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 (S)-2-haloacid + H2O
-
 Azotobacter sp. RC26 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Azotobacter sp. lactate + HCl
-
 ?
3.8.1.2 2-chloropropionate + H2O
-
 Azotobacter sp. RC26 lactate + HCl
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 L-2-haloalkanoic acid + H2O
-
 Azotobacter sp. RC26 D-2-hydroxyalkanoic acid + halide
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Azotobacter sp. glycolate + HCl
-
 ?
3.8.1.2 monochloroacetate + H2O
-
 Azotobacter sp. RC26 glycolate + HCl
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.2 monomer 1 * 29000 SDS-PAGE Azotobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.8.1.2 30
-
-
 Azotobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.8.1.2 20 60
-
 Azotobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.8.1.2 60
-
 activity after 30 min reduced by 50% Azotobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.2 8 9
-
 Azotobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.8.1.2 Azotobacter sp. isoelectric focusing
-
 5.5