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Literature summary extracted from

  • Soda, K.; Kurihara, T.; Liu, J.Q.; Nardi-Dei, V.; Park, C.; Miyagi, M.; Tsunasawa, S.; Esaki, N.
    Bacterial 2-haloacid dehalogenases: Structures and catalytic properties (1996), Pure Appl. Chem., 68, 2097-2103.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.8.1.10 additional information not: 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide, Woodward reagent K Pseudomonas sp.
3.8.1.11 N-ethylmaleimide
-
 Pseudomonas putida
3.8.1.11 p-chloromercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.10 1.1
-
 L-2-chloropropionate
-
 Pseudomonas sp.
3.8.1.10 3.2
-
 DL-2-chloropropionate
-
 Pseudomonas sp.
3.8.1.10 4.8
-
 D-2-chloropropionate
-
 Pseudomonas sp.
3.8.1.10 5
-
 Monochloroacetate
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.8.1.9 33601
-
 4 * 33601 Pseudomonas putida
3.8.1.9 135000
-
-
 Pseudomonas putida
3.8.1.10 68000
-
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.8.1.9 (R)-2-haloacid + H2O Pseudomonas putida
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O Rhizobium sp.
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O Pseudomonas putida PP3
-
 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O Pseudomonas putida AJ1/23
-
 (S)-2-hydroxyacid + halide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.9 Pseudomonas putida
-
-
-
3.8.1.9 Pseudomonas putida AJ1/23
-
-
-
3.8.1.9 Rhizobium sp.
-
-
-
3.8.1.10 Pseudomonas putida
-
-
-
3.8.1.10 Pseudomonas putida PP3
-
-
-
3.8.1.10 Pseudomonas sp.
-
-
-
3.8.1.10 Pseudomonas sp. 113
-
-
-
3.8.1.10 Rhizobium sp.
-
-
-
3.8.1.11 Pseudomonas putida
-
 PP3
-
3.8.1.11 Pseudomonas putida PP3
-
 PP3
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.9 (R)-2-haloacid + H2O
-
 Pseudomonas putida (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O
-
 Rhizobium sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O
-
 Pseudomonas putida PP3 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.9 (R)-2-haloacid + H2O
-
 Pseudomonas putida AJ1/23 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas putida (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Rhizobium sp. (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas putida PP3 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (R)-2-haloacid + H2O
-
 Pseudomonas putida AJ1/23 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas putida (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Rhizobium sp. (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas sp. 113 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas putida PP3 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 (S)-2-haloacid + H2O
-
 Pseudomonas putida AJ1/23 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.10 D-2-monochloropropionate + H2O
-
 Pseudomonas sp. L-lactate + HCl
-
 ?
3.8.1.10 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. DL-lactate + HCl
-
 ?
3.8.1.10 DL-2-chloropropionate + H2O
-
 Pseudomonas sp. 113 DL-lactate + HCl
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. glycolate + HCl
-
 ?
3.8.1.10 monochloroacetate + H2O
-
 Pseudomonas sp. 113 glycolate + HCl
-
 ?
3.8.1.10 trichloroacetate + H2O
-
 Pseudomonas sp. ?
-
 ?
3.8.1.10 trichloroacetate + H2O
-
 Pseudomonas sp. 113 ?
-
 ?
3.8.1.11 (R)-2-haloacid + H2O
-
 Pseudomonas putida (R)-2-hydroxyacid + halide
-
 ?
3.8.1.11 (R)-2-haloacid + H2O
-
 Pseudomonas putida PP3 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.11 (R)-2-haloacid + H2O
-
 Pseudomonas putida AJ1/23 (R)-2-hydroxyacid + halide
-
 ?
3.8.1.11 (S)-2-haloacid + H2O
-
 Pseudomonas putida (S)-2-hydroxyacid + halide
-
 ?
3.8.1.11 (S)-2-haloacid + H2O
-
 Pseudomonas putida PP3 (S)-2-hydroxyacid + halide
-
 ?
3.8.1.11 (S)-2-haloacid + H2O
-
 Pseudomonas putida AJ1/23 (S)-2-hydroxyacid + halide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.9 tetramer 4 * 33601 Pseudomonas putida
3.8.1.10 dimer
-
 Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8.1.9 9.5
-
-
 Pseudomonas putida
3.8.1.10 9.5
-
-
 Pseudomonas sp.