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Literature summary extracted from

  • Finn, R.F.; Leimgruber, R.M.; Boyle, D.M.; Jennings, M.G.; Kimack, N.M.; Smith, C.E.; Bishop, B.F.; Frazier, R.B.; Magin, K.M.; Fuchs, R.L.; Reed, A.J.
    Purification and biochemical comparison of 1-aminocyclopropane-1-carboxylic acid deaminase proteins expressed in delayed ripening tomato and Escherichia coli: studies for a food safety assessment (1996), J. Agric. Food Chem., 44, 381-387.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.99.7 agriculture development of tomato plants with delayed fruit ripening by Agrobacterium tumefaciens mediated transfer of a gene encoding 1-aminocyclopropane-1-carboxylic acid deaminase, the enzyme delays fruit ripening by decreasing ethylene synthesis Pseudomonas chlororaphis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.99.7 expression in Escherichia coli and in delayed ripening tomato Pseudomonas chlororaphis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.99.7 36500
-
 x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas chlororaphis
3.5.99.7 36800
-
 x * 36800, calculation from amino acid composition Pseudomonas chlororaphis

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.7 Pseudomonas chlororaphis
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.99.7 no modification the enzyme expressed in Escherichia coli and in delayed ripening tomatoes is not glycosylated Pseudomonas chlororaphis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.7 expressed in delayed ripening tomato plants Pseudomonas chlororaphis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.99.7 0.36
-
 expressed in delayed ripening tomatoes, 37°C Pseudomonas chlororaphis

Storage Stability

EC Number Storage Stability Organism
3.5.99.7 -80°C, pH 7.5, 8 mg/ml protein, stable for at least 6 months Pseudomonas chlororaphis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.7 1-aminocyclopropane-1-carboxylate + H2O
-
 Pseudomonas chlororaphis 2-oxobutanoate + NH3
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.99.7 ? x * 36500, enzyme expressed in Escherichia coli, SDS-PAGE Pseudomonas chlororaphis
3.5.99.7 ? x * 36800, calculation from amino acid composition Pseudomonas chlororaphis