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Literature summary extracted from

  • Lara-Gonzalez, S.; Dixon, H.B.; Mendoza-Hernandez, G.; Altamirano, M.M.; Calcagno, M.L.
    On the role of the N-terminal group in the allosteric function of glucosamine-6-phosphate deaminase from Escherichia coli (2000), J. Mol. Biol., 301, 219-227.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.99.6 N-acetylglucosamine 6-phosphate
-
Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.6 additional information
-
additional information comparison of KM of unmodified and N-terminal modified enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.6 Escherichia coli P0A759
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.5.99.6 side-chain modification enzyme is chemically transaminated, modifying its N-terminal methionine residue to a 2-oxo-4-(methylthio)butyryl group, the transamination markedly reduces the affinity of the enzyme for its allosteric activator N-acetylglucosamine 6-phosphate, in contrast with the unmodified enzyme, which behaves as a typical allosteric K-enzyme, the modified enzyme becomes a mixed K-V allosteric protein, borohydride reduction to obtain the corresponding enzyme with a terminal hydroxy group, this enzyme shows significant recovery of the catalytic catalytic activity and its allosteric activation pattern, becomes similar to that found for the unmodified enzyme Escherichia coli
3.5.99.6 side-chain modification the modification by diethyl dicarbonate results in complete inactivation of enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.6 D-glucosamine 6-phosphate + H2O
-
Escherichia coli D-fructose 6-phosphate + NH3
-
?

Subunits

EC Number Subunits Comment Organism
3.5.99.6 hexamer
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.99.6 additional information
-
additional information comparison of kcat of unmodified and N-terminal modified enzyme Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.99.6 additional information
-
effect of pH on homotropic co-operativity in the native and modified deaminases Escherichia coli