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Literature summary extracted from

  • Altamirano, M.M.; Plumbridge, J.A.; Horjales, H.; Calcagno, M.L.
    Asymmetric allosteric activation of Escherichia coli glucosamine-6-phosphate deaminase produced by replacement of Tyr121 (1995), Biochemistry, 34, 6074-6082.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.5.99.6 C228S the kinetic and allosteric properties of the mutant enzyme in which Ser replaces Cys219 or Cys228 are the same as those described for the wild-type enzyme. The same result is obtained with the double mutation Escherichia coli
3.5.99.6 Y121T while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate Escherichia coli
3.5.99.6 Y121W while the wild-type enzyme behaves as a classical allosteric K-system which can be described by the allosteric concerted model, the mutant forms Y121T and Y121W present an asymmetric behaviour towards the allosteric activator, which can be described as two distinct half-of-the-sites allosteric activation steps occuring with different affinities for the N-acetyl-D-glucosamine 6-phosphate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.99.6 0.67
-
 D-glucosamine 6-phosphate pH 8.0, 30°C, mutant enzyme Tyr121Trp Escherichia coli
3.5.99.6 2.01
-
 D-glucosamine 6-phosphate pH 8.0, 30°C, wild-type enzyme Escherichia coli
3.5.99.6 2.6
-
 D-glucosamine 6-phosphate pH 8.0, 30°C, mutant enzyme Tyr121Thr Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.6 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.6 D-glucosamine 6-phosphate + H2O
-
 Escherichia coli D-fructose 6-phosphate + NH3
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.99.6 additional information
-
 additional information
-
 Escherichia coli