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Literature summary extracted from

  • Kikuchi, K.; Kikuchi, H.
    Stabilization and purification of glucosamine 6-phosphate isomerase from rat kidney (1979), Sci. Rep. Res. Inst. Tohoku Univ. Med., 26, 92-98.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.5.99.6 D-glucosamine 6-phosphate and N-acetylglucosamine 6-phosphate stabilize against heat inactivation at 60°C, and protect the enzyme from tryptic digestion Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.99.6 PCMB protection by 0.3 mM glucosamine 6-phosphate, no protection by 0.3 mM N-acetylglucosamine 6-phosphate Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.99.6 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.99.6
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.99.6 kidney
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
3.5.99.6 -70°C, stable for 70 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.99.6 D-glucosamine 6-phosphate + H2O
-
 Rattus norvegicus D-fructose 6-phosphate + NH3
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.99.6 60
-
 10 min, in presence of 1.7 mM glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate, 7% loss of activity Rattus norvegicus
3.5.99.6 60
-
 90% loss of activity within 1 min without stabilizer, stable for 1 min in presence of 0.6 mM glucosamine 6-phosphate or N-acetylglucosamine 6-phosphate Rattus norvegicus
3.5.99.6 65
-
 complete loss of activity Rattus norvegicus