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Literature summary extracted from
- Purification of glucosamine 6-phosphate deaminase from human brain (1961), Biochim. Biophys. Acta, 54, 273-283.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | PCMB | inhibits at high concentrations, activates at low concentrations | Homo sapiens |  |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | Cd2+ | - |
Homo sapiens | |
| 3.5.99.6 | cysteine | - |
Homo sapiens | |
| 3.5.99.6 | diphosphate | - |
Homo sapiens | |
| 3.5.99.6 | EDTA | - |
Homo sapiens | |
| 3.5.99.6 | PCMB | inhibits at high concentrations, activates at low concentrations | Homo sapiens | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.99.6 | 0.25 | - |
D-glucosamine 6-phosphate | pH 8.5, 37°C, in presence of N-acetylglucosamine 6-phosphate and Mn2+ | Homo sapiens | |
| 3.5.99.6 | 2.37 | - |
D-glucosamine 6-phosphate | pH 8.5, 37°C, without activator | Homo sapiens | |
| 3.5.99.6 | 3.5 | - |
D-fructose 6-phosphate | pH 8.5, 37°C, in presence of N-acetylglucosamine 6-phosphate and Mn2+ | Homo sapiens | |
| 3.5.99.6 | 16 | - |
NH4+ | pH 8.5, 37°C, in presence of N-acetylglucosamine 6-phosphate and Mn2+ | Homo sapiens | |
| 3.5.99.6 | 22.5 | - |
D-fructose 6-phosphate | pH 8.5, 37°C, in absence of activators | Homo sapiens | |
| 3.5.99.6 | 25 | - |
NH4+ | in absence of activators | Homo sapiens | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.99.6 | Co2+ | activates | Homo sapiens | |
| 3.5.99.6 | Hg2+ | activates | Homo sapiens | |
| 3.5.99.6 | Mg2+ | activates | Homo sapiens | |
| 3.5.99.6 | Mn2+ | activates, reverses inhibition by EDTA | Homo sapiens | |
| 3.5.99.6 | additional information | Mn2+, Hg2+, Mg2+, Co2+ and Ni2+ enhance in decreasing order the activity in both directions | Homo sapiens | |
| 3.5.99.6 | Ni2+ | activates | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | Homo sapiens | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.99.6 | - |
Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.5.99.6 | brain | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | additional information | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.99.6 | D-glucosamine 6-phosphate + H2O | - |
Homo sapiens | D-fructose 6-phosphate + NH3 | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 8.5 | 8.7 | - |
Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.99.6 | 7 | 9.5 | pH 7.0: about 55% of maximal activity, pH 9.5: about 74% of maximal activity | Homo sapiens |
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Release 2023.1 (January 2023)
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