EC Number | Cloned (Comment) | Organism |
---|---|---|
3.5.4.13 | expression in Escherichia coli | Methanocaldococcus jannaschii |
3.6.1.23 | expressed in Escherichia coli BL21 | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.4.13 | apoenzyme in complex with dCTP and with dUTP | Methanocaldococcus jannaschii |
3.5.4.30 | apo form complexed with dCTP and dUTP, hanging drop vapor diffusion method | Methanocaldococcus jannaschii |
3.6.1.23 | hanging drop vapor-diffusion method | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | dTTP | competitive feedback inhibition | Methanocaldococcus jannaschii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | additional information | - |
additional information | lower affinity for dUTP than strict dUTPases | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.4.30 | 77900 | - |
enzyme with addition of six histidines at the N-terminus, gel filtration | Methanocaldococcus jannaschii |
3.6.1.23 | 136000 | - |
gel filtration | Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.30 | dCTP + H2O | Methanocaldococcus jannaschii | first step in conversion of dCTP to dTTP | dUMP + diphosphate + NH3 | - |
? | |
3.6.1.23 | dUTP + H2O | Methanocaldococcus jannaschii | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.4.13 | Methanocaldococcus jannaschii | Q57872 | bifunctional enzyme dCTP deaminase-dUTP nucleotidohydrolase | - |
3.5.4.30 | Methanocaldococcus jannaschii | Q57872 | - |
- |
3.6.1.23 | Methanocaldococcus jannaschii | Q57872 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.4.30 | - |
Methanocaldococcus jannaschii |
3.6.1.23 | recombinant protein | Methanocaldococcus jannaschii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.5.4.13 | dCTP + H2O = dUTP + NH3 | mechanism of bifunctional enzyme activity and feedback inhibition by dTTP | Methanocaldococcus jannaschii |
EC Number | Storage Stability | Organism |
---|---|---|
3.6.1.23 | -70°C, 50 mM HEPES, pH 7.2, 150 mM NaCl, 2 mM dithiothreitol | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.4.30 | dCTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate + NH3 | - |
? | |
3.5.4.30 | dCTP + H2O | first step in conversion of dCTP to dTTP | Methanocaldococcus jannaschii | dUMP + diphosphate + NH3 | - |
? | |
3.5.4.30 | additional information | enzyme has also dUTP diphosphatase activity, dCTP deamination and dUTP diphosphatase activities occur independently at the same active site | Methanocaldococcus jannaschii | ? | - |
? | |
3.6.1.23 | dTTP + H2O | - |
Methanocaldococcus jannaschii | dTMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Methanocaldococcus jannaschii | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA, provides the substrate thymidylate synthase and is therefore essential for the synthesis of dTTP | Methanocaldococcus jannaschii | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.4.13 | hexamer | crystallization data | Methanocaldococcus jannaschii |
3.5.4.30 | hexamer | two trimers can assemble into a hexamer by stacking on top of each other | Methanocaldococcus jannaschii |
3.5.4.30 | trimer | two trimers can assemble into a hexamer by stacking on top of each other | Methanocaldococcus jannaschii |
3.6.1.23 | hexamer | two trimers assemble into a hexamer, crystal structure analysis | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.23 | DCD-DUT | catalyzes the deamination of dCTP and the dephosphorylation of dUTP without releasing an intermediate | Methanocaldococcus jannaschii |