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Literature summary extracted from

  • Dutka, S.; Meinnel, T.; Lazennec, C.; Mechulam, Y.; Blanquet, S.
    Role of the 1-72 base pair in tRNAs for the activity of Escherichia coli peptidyl-tRNA hydrolase (1993), Nucleic Acids Res., 21, 4025-4030.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.29 additional information
-
 additional information effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the Km-value Escherichia coli
3.1.1.29 0.0022
-
 N-acetyl-Met-tRNA pH 7.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.29 Mn2+ the enzyme almost completely inactive in absence of divalent cations. Mn2+ is partly effective, optimal concentration is about 0.2-0.5 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Escherichia coli
-
 construction of a bacterial strain overexpressing the enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 Met-tRNAMet + H2O
-
 Escherichia coli Met + tRNAMet
-
 ?
3.1.1.29 additional information derivatives of tRNAMetf with various combinations of bases at position 1 and 72 in the acceptor stem have been produced, aminoacylated and chemically acetylated. TrNAmetd derivatives with either C1A72, c1C72, U1G72, U1C72 or A1C72 behave as poor substrates of the enzyme compared to those with C1G72, U1A72, G1C72, A1U72 or G1U72 Escherichia coli ?
-
 ?
3.1.1.29 N-acetyl-Met-tRNA + H2O
-
 Escherichia coli N-acetyl-Met + tRNA
-
 ?
3.1.1.29 Val-tRNAVal + H2O
-
 Escherichia coli Val + tRNAVal
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.29 additional information
-
 additional information effect of mutations altering the 1-72 pair of E. coli tRNAMetf on the turnover-number Escherichia coli
3.1.1.29 0.7
-
 N-acetyl-Met-tRNA pH 7.0, 37°C Escherichia coli

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.29 Escherichia coli isoelectric focusing
-
 10